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Titolo:
Endogenously generated nitric oxide by nitric-oxide synthase gene transferinhibits cellular proliferation
Autore:
Maeda, Y; Ikeda, U; Oya, K; Shimpo, M; Ueno, S; Okada, K; Saito, T; Mano, H; Ozawa, K; Shimada, K;
Indirizzi:
Jichi Med Sch, Dept Cardiol, Inst Hematol, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Jichi Med Sch, Dept Endocrinol & Metab, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Jichi Med Sch, Dept Mol Biol, Minami Kawachi, Tochigi 3290498, Japan JichiMed Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan
Titolo Testata:
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
fascicolo: 1, volume: 292, anno: 2000,
pagine: 387 - 393
SICI:
0022-3565(200001)292:1<387:EGNOBN>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; RABBIT CAROTID ARTERIES; PROTEIN-KINASE-C; ENDOTHELIN RECEPTOR; BALLOON ANGIOPLASTY; CARDIAC MYOCYTES; RAT; EXPRESSION; CLONING; SUBTYPE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Ikeda, U Jichi Med Sch, Dept Cardiol, Inst Hematol, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 290498, Japan
Citazione:
Y. Maeda et al., "Endogenously generated nitric oxide by nitric-oxide synthase gene transferinhibits cellular proliferation", J PHARM EXP, 292(1), 2000, pp. 387-393

Abstract

We investigated whether endothelial nitrite oxide synthase (NOS) gene transfer inhibited cellular proliferation. Endothelial NOS and endothelin type A receptor genes were transferred into 293 cells, a human embryonic kidney cell line, by calcium-phosphate coprecipitation. The cytosolic free Ca2+ levels ([Ca2+](i)) of transfected cells were estimated with fura-2 fluorescence. Thymidine incorporation was increased by endothelin-1 in type A receptor-transfected cells. The endothelial NOS gene transfer did not affect endothelin-1-induced increase in [Ca2+](i) of type A receptor-transfected cells,but markedly inhibited mitogen-activated protein kinase and c-fos promoteractivities. The endothelial NOS gene transfer also inhibited thymidine incorporation into type A receptor-transfected cells in response to endothelin-1, which was abolished in the presence of the NOS inhibitor N-G-monomethyl-L-arginine acetate. The endothelin-1-induced increase in cell number was significantly suppressed by endothelial NOS gene transfer as well as by the mitogen-activated protein kinase inhibitor PD98059. These results indicate that endothelial NOS gene transfer inhibits cellular proliferation via inhibition of the mitogen-activated protein kinase cascade.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 09:44:23