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Titolo:
An essential role of interleukin-1 beta in mediating NF-kappa B activity and COX-2 transcription in cells of the blood-brain barrier in response to asystemic and localized inflammation but not during endotoxemia
Autore:
Laflamme, N; Lacroix, S; Rivest, S;
Indirizzi:
Univ Laval, Ctr Rech, Mol Endocrinol Lab, Quebec City, PQ G1V 4G2, Canada Univ Laval Quebec City PQ Canada G1V 4G2 Quebec City, PQ G1V 4G2, Canada Univ Laval, Dept Anat & Physiol, Quebec City, PQ G1V 4G2, Canada Univ Laval Quebec City PQ Canada G1V 4G2 Quebec City, PQ G1V 4G2, Canada
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 24, volume: 19, anno: 1999,
pagine: 10923 - 10930
SICI:
0270-6474(199912)19:24<10923:AEROIB>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLOOXYGENASE-2 MESSENGER-RNA; VASCULAR ENDOTHELIAL-CELLS; RAT-BRAIN; EXPRESS CYCLOOXYGENASE-2; INDUCED FEVER; LIPOPOLYSACCHARIDE; INDUCTION; MICE; DEFICIENT; GENES;
Keywords:
blood vessels; circumventricular organs; endothelial cells; in situ hybridization histochemistry; inflammation; interleukin-1 beta-deficient mice; immunocytochemistry; lipopolysaccharide; proinflammatory cytokines; microglia; macrophages; septic shock; transcription factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Rivest, S Univ Laval, Ctr Rech, Mol Endocrinol Lab, 2705 Blvd Laurier, Quebec City, PQ G1V 4G2, Canada Univ Laval 2705 Blvd Laurier Quebec City PQ Canada G1V 4G2 anada
Citazione:
N. Laflamme et al., "An essential role of interleukin-1 beta in mediating NF-kappa B activity and COX-2 transcription in cells of the blood-brain barrier in response to asystemic and localized inflammation but not during endotoxemia", J NEUROSC, 19(24), 1999, pp. 10923-10930

Abstract

When released into the bloodstream, proinflammatory cytokines have the ability to trigger the transcription of different genes in cells of the blood-brain barrier (BBB), including members of the nuclear factor kappa B (NF-kappa B) family and cyclooxygenase-2 (COX-2), the limiting enzyme for the formation of prostaglandins (PGs). The present study investigated the possibility that interleukin-1 beta (IL-1 beta) plays an essential role in these events during a systemic inflammatory response. Both wild-type and IL-1 beta-deficient mice were killed at different times after two different immunogenic stimuli, i.e., intraperitoneal lipopolysaccharide (LPS) injection and intramuscular turpentine injection, used here as a model of systemic localized inflammatory insult. The inhibitory factor kappa B alpha (I kappa B alpha, index of NF-kappa B activity) and COX-2 transcripts were detected throughout the brain by means of in situ hybridization. Systemic LPS injection caused a strong and rapid expression of I kappa B alpha in endothelial cells lining the BBB of large and small blood vessels and thereafter within parenchymal microglia across the brain. This treatment also provoked a transient expression of COX-2 along cells of the vascular system, and the expression pattern and intensity of the signal for both transcripts were essentially the same in wild-type and IL-1 beta-deficient animals. In contrast, the induction of these genes that was quite selective to the cells of the BBB in response to intramuscularly turpentine insult was completely abolished in IL-1 beta-deficient mice. Indeed, a late and prolonged expression of I kappa Balpha and COX-2 mRNAs was found along the cerebral blood vessels in response to the sterile and localized inflammation in wild-type mice, whereas such induction was absent in the brain of IL-1 beta-deficient animals. These results indicate that IL-1 beta has an obligatory role in the activation of NF-kappa B molecules and PGs within endothelial cells of the BBB in an experimental model of intramuscularly turpentine-induced inflammation but not during endotoxemia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 18:27:29