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Titolo:
Dendritic cells genetically engineered to express Fas ligand induce donor-specific hyporesponsiveness and prolong allograft survival
Autore:
Min, WP; Gorczynski, R; Huang, XY; Kushida, M; Kim, P; Obataki, M; Lei, J; Suri, RM; Cattral, MS;
Indirizzi:
Toronto Hosp, Dept Surg, Toronto, ON M5G 2C4, Canada Toronto Hosp TorontoON Canada M5G 2C4 Surg, Toronto, ON M5G 2C4, Canada Toronto Hosp, Multiorgan Transplant Program, Toronto, ON M5G 2C4, Canada Toronto Hosp Toronto ON Canada M5G 2C4 ogram, Toronto, ON M5G 2C4, Canada Univ Toronto, Res Inst, Toronto, ON, Canada Univ Toronto Toronto ON Canada iv Toronto, Res Inst, Toronto, ON, Canada Univ Toronto, Hosp Sick Children, Toronto, ON M5G 1X8, Canada Univ Toronto Toronto ON Canada M5G 1X8 ldren, Toronto, ON M5G 1X8, Canada
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 1, volume: 164, anno: 2000,
pagine: 161 - 167
SICI:
0022-1767(20000101)164:1<161:DCGETE>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; TUMOR-ASSOCIATED ANTIGEN; MOUSE BONE-MARROW; T-CELLS; INDUCED APOPTOSIS; GENE-TRANSFER; MIGRATION PATTERNS; ANTITUMOR IMMUNITY; ISLET ALLOGRAFTS; TNF-ALPHA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Cattral, MS Toronto Hosp, Dept Surg, NU10-145,621 Univ Ave, Toronto, ON M5G 2C4, Canada Toronto Hosp NU10-145,621 Univ Ave Toronto ON Canada M5G 2C4 a
Citazione:
W.P. Min et al., "Dendritic cells genetically engineered to express Fas ligand induce donor-specific hyporesponsiveness and prolong allograft survival", J IMMUNOL, 164(1), 2000, pp. 161-167

Abstract

Polarization of an immune response toward tolerance or immunity is dictated by the interactions between T cells and dendritic cells (DC), which in turn are modulated by the expression of distinct cell surface molecules, and the cytokine milieu in which these interactions are taking place. Genetic modification of DC with genes coding for specific immunoregulatory cell surface molecules and cytokines offers the potential of inhibiting immune responses by selectively targeting Ag-specific T cells. In this study, the immunomodulatory effects of transfecting murine bone marrow-derived DC with Fas ligand (FasL) were investigated. In this study, we show that Fast transfection of DC markedly augmented their capacity to induce apoptosis of Fas(+) cells. FasL-transfected DC inhibited allogeneic MLR in vitro, and induced hyporesponsiveness to alloantigen in vivo. The induction of hyporesponsiveness was Ag specific and was dependent on the interaction between Fast on DC and Fas on T cells. Finally, we show that transfusion of FasL-DC significantly prolonged the survival of fully MHC-mismatched vascularized cardiac allografts, Our findings suggest that DC transduced with,FasL may facilitate the development of Ag-specific unresponsiveness for the prevention of organ rejection. Moreover, they highlight the potential of genetically engineeringDC to express other genes that affect immune responses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 02:44:52