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Titolo:
Protein kinase C epsilon IS required for the induction of mitogen-activated protein kinase phosphatase-1 in lipopolysaccharide-stimulated macrophages
Autore:
Valledor, AF; Xaus, J; Comalada, M; Soler, C; Celada, A;
Indirizzi:
Univ Barcelona, Fac Biol, Dept Fisiol, E-08028 Barcelona, Spain Univ Barcelona Barcelona Spain E-08028 Fisiol, E-08028 Barcelona, Spain Univ Barcelona, Fdn August Pi & Sunyer, E-08028 Barcelona, Spain Univ Barcelona Barcelona Spain E-08028 Sunyer, E-08028 Barcelona, Spain
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 1, volume: 164, anno: 2000,
pagine: 29 - 37
SICI:
0022-1767(20000101)164:1<29:PKCEIR>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; MURINE PERITONEAL-MACROPHAGES; SMOOTH-MUSCLE CELLS; P70 S6 KINASE; BACTERIAL LIPOPOLYSACCHARIDE; TYROSINE-PHOSPHATASE; SIGNAL-TRANSDUCTION; HUMAN MONOCYTES; GENE-EXPRESSION; GROWTH-FACTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
71
Recensione:
Indirizzi per estratti:
Indirizzo: Celada, A Univ Barcelona, Fac Biol, Dept Fisiol, Av Diagonal 645,Campus Vellvitge, E-08028 Barcelona, Spain Univ Barcelona Av Diagonal 645,Campus Vellvitge Barcelona Spain E-08028
Citazione:
A.F. Valledor et al., "Protein kinase C epsilon IS required for the induction of mitogen-activated protein kinase phosphatase-1 in lipopolysaccharide-stimulated macrophages", J IMMUNOL, 164(1), 2000, pp. 29-37

Abstract

LPS induces in bone marrow macrophages the transient expression of mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1). Because MKP-1 playsa crucial role in the attenuation of different MAPK cascades, we were interested in the characterization of the signaling mechanisms involved in the control of MKP-1 expression in LPS-stimulated macrophages, The induction ofMKP-1 was blocked by genistein, a tyrosine kinase inhibitor, and by two different protein kinase C (PKC) inhibitors (GF109203X and calphostin C), We had previously shown that bone marrow macrophages express the isoforms PKC beta I, epsilon, and zeta. Of all these, only PKC beta I and epsilon are inhibited by GF109203X, The following arguments suggest that PKC epsilon is required selectively for the induction of MKP-1 by LPS, First, in macrophages exposed to prolonged treatment with PMA, MKP-1 induction by LPS correlates with the levels of expression of PKC epsilon but not with that of PKC beta I, Second, Go6976, an inhibitor selective for conventional PKCs, including PKC beta I, does not alter MKP-1 induction by LPS, Last, antisense oligonucleotides that block the expression of PKC epsilon, but not those selective for PKC beta I or PKC zeta, inhibit MKP-1 induction and lead to an increase of extracellular-signal regulated kinase activity during the macrophage response to LPS, Finally, in macrophages stimulated with LPS we observed significant activation of PKC epsilon, In conclusion, our results demonstratean important role for PKC epsilon in the induction of MKP-1 and the subsequent negative control of MAPK activity in macrophages.

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Documento generato il 05/07/20 alle ore 10:04:30