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Titolo:
Liquid chromatographic analysis and preliminary pharmacokinetics of methotrexate in cancer patients co-treated with docetaxel
Autore:
Sparreboom, A; Loos, WJ; Nooter, K; Stoter, G; Verweij, J;
Indirizzi:
Rotterdam Canc Inst, Daniel den Hoed Klin, Dept Med Oncol, Lab Expt Chemotherapy & Pharmacol, NL-3008 AE Rotterdam, Netherlands Rotterdam Canc Inst Rotterdam Netherlands NL-3008 AE terdam, Netherlands Univ Rotterdam Hosp, NL-3008 AE Rotterdam, Netherlands Univ Rotterdam Hosp Rotterdam Netherlands NL-3008 AE terdam, Netherlands
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 735, anno: 1999,
pagine: 111 - 119
SICI:
1387-2273(19991126)735:1<111:LCAAPP>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-DOSE METHOTREXATE; SOLID-PHASE EXTRACTION; HUMAN-PLASMA; METABOLITES 7-HYDROXYMETHOTREXATE; PRECOLUMN DERIVATIZATION; BIOLOGICAL-FLUIDS; CHEMOTHERAPY; IMMUNOASSAY; LEUKEMIA; URINE;
Keywords:
methotrexate; docetaxel;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Sparreboom, A Rotterdam Canc Inst, Dept Med Oncol, Groene Hilledrijk 301, NL-3075 EA Rotterdam, Netherlands Rotterdam Canc Inst Groene Hilledrijk 301Rotterdam Netherlands NL-3075 EA
Citazione:
A. Sparreboom et al., "Liquid chromatographic analysis and preliminary pharmacokinetics of methotrexate in cancer patients co-treated with docetaxel", J CHROMAT B, 735(1), 1999, pp. 111-119

Abstract

A new HPLC method has been developed for the quantitative determination ofmethotrexate (MTX) and its 7-hydroxyl metabolite-inhuman plasma. Samples were purified by protein precipitation with acetone and methanol, and a sample clean-up with a mixture of n-butanol and diethyl ether. The analytes were separated on an RP Inertsil ODS-80A column and eluted in a solvent systemcontaining 5% (v/v) tetrahydrofuran in water (pH 2.0). UV absorption measurement was performed at 313 nm, and the detector response was linear in a concentration range of 10-10 000 ng/ml. The lower limit of quantitation of-MTX was 10 ng/ml using 1 ml sample aliquots. Values for accuracy and (within-run and between-run) precision were between 95.5-111% and 3.69-11.0%, respectively, at four concentrations analyzed in quintuplicate on four separateoccasions. The assay was applied to study the effects of docetaxel co-administration on the pharmacokinetics and metabolism of MTX in cancer patients. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:01:12