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Titolo:
Regulation of arachidonic acid mobilization in lipopolysaccharide-activated P388D(1) macrophages by adenosine triphosphate
Autore:
Balboa, MA; Balsinde, J; Johnson, CA; Dennis, EA;
Indirizzi:
Univ Calif San Diego, Dept Biochem & Chem, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 & Chem, La Jolla, CA 92093 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 51, volume: 274, anno: 1999,
pagine: 36764 - 36768
SICI:
0021-9258(199912)274:51<36764:ROAAMI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOSPHOLIPASE A(2) ENZYMES; EXPRESSION; RECEPTOR; ATP; CELLS; MODULATION; P2X(7); PROSTAGLANDIN-E(2); INHIBITION; DEATH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Dennis, EA Univ Calif San Diego, Dept Biochem & Chem, 4080 BSB M-C 0601,9500 Gilman Dr, La Jolla, CA 92093 USA Univ Calif San Diego 4080 BSB M-C 0601,9500 Gilman Dr La Jolla CA USA 92093
Citazione:
M.A. Balboa et al., "Regulation of arachidonic acid mobilization in lipopolysaccharide-activated P388D(1) macrophages by adenosine triphosphate", J BIOL CHEM, 274(51), 1999, pp. 36764-36768

Abstract

Murine P388D, macrophages exhibit a delayed prostaglandin biosynthetic response when exposed to bacterial lipopolysaccharide (LPS) for prolonged periods of time that is dependent on induction of the genes coding for Group V secretory phospholipase A(2) and cyclooxygenase-2, We herein report that LPS-induced arachidonic acid (AA) metabolite release in P388D(1) macrophages is strongly attenuated by the P2X(7) purinergic receptor antagonists periodate-oxidized ATP and pyridoxal-phosphate-6-azophenyl-2',4'-disulfonic acid,and this is accompanied by suppression of the expression of both Group V secretory phospholipase A(2) and cyclooxygenase-2, The effect appears to be specific for LPS, because the P2X(7) purinergic receptor antagonists do notaffect P388D, cell stimulation by other stimuli such as platelet-activating factor or the Ca2+ ionophore A23187. Moreover, extracellular nucleotides are found to stimulate macrophage AA mobilization with a pharmacological profile that implicates the participation of the P2X(7) receptor and that is inhibited by periodate-oxidized ATP. Collectively these results demonstratecoupling of the P2X(7) receptor to the AA cascade in P388D, macrophages and implicate the participation of this type of receptor in LPS-induced AA mobilization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 09:55:33