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Titolo:
Contribution of Escherichia coli alpha-hemolysin to bacterial virulence and to intraperitoneal alterations in peritonitis
Autore:
May, AK; Gleason, TG; Sawyer, RG; Pruett, TL;
Indirizzi:
Univ Virginia, Surg Infect Dis Lab, Charlottesville, VA USA Univ VirginiaCharlottesville VA USA ct Dis Lab, Charlottesville, VA USA
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 1, volume: 68, anno: 2000,
pagine: 176 - 183
SICI:
0019-9567(200001)68:1<176:COECAT>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTRAINTESTINAL INFECTIONS; ERYTHROCYTE-MEMBRANES; BINDING; STRAINS; RELEASE; INVITRO; CELLS; HEMAGGLUTINATION; PATHOGENICITY; GRANULOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: May, AK Univ Alabama, 112 LHRB,701 S 19th St, Birmingham, AL 35294 USA Univ Alabama 112 LHRB,701 S 19th St Birmingham AL USA 35294 94 USA
Citazione:
A.K. May et al., "Contribution of Escherichia coli alpha-hemolysin to bacterial virulence and to intraperitoneal alterations in peritonitis", INFEC IMMUN, 68(1), 2000, pp. 176-183

Abstract

Alpha-hemolysin (Hly) is a common exotoxin produced by Escherichia coli that enhances virulence in a number of clinical infections. The addition of hemolysin production to laboratory bacterial strains is known to increase the lethality of E. coil peritonitis. However, the mechanisms involved have not been determined and the contribution of hemolysin to the alterations in the host intraperitoneal environment and the leukocyte response is not known, Utilizing a rat peritonitis model, we show that wild-type hemolytic E. coli strains have a significant competitive advantage over nonhemolytic strains within the peritoneum. To examine the specific contribution of Hly to E. coli-induced virulence and alterations within the peritoneum, a mixed peritonitis model off. coli, Bacteroides fragilis, and sterile fecal adjuvant was used. Three transformed E. coli strains mere utilized: one strongly secretes active hemolysin (WAF 270), a second secretes active hemolysin but a reduced amount (WAF 260), and the third does not produce hemolysin (WAF 108). After an equal inoculum of each of the three strains, WAF 270 produced amarkedly increased lethality and an increased recovery of both E. coli andB. fragilis from the host relative to the other strains. Changes in the intraperitoneal pH, degree of erythrocyte lysis, and recruitment and viability of leukocytes within the peritoneum following the induction of peritonitis differed significantly between the strongly hemolytic and nonhemolytic strains. Induction of peritonitis with WAF 270 caused a pronounced decrease in intraperitoneal pH, lysis of most of the intraperitoneal erythrocytes, and a marked decrease in recoverable viable leukocytes compared to WAF 108. Thus, hemolysin production by E. coil within the peritoneum may alter not only the host's ability to control the hemolytic strain itself but also otherorganisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 11:56:42