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Titolo:
Ultrastructural and functional studies of the interaction between IL-12 and IL-2 for the generation of lymphokine-activated killer cells
Autore:
Facchetti, P; Tacchetti, C; Prigione, I; Airoldi, I; Favre, A; Grossi, CE; Pistoia, V;
Indirizzi:
Ist Giannina Gaslini, Lab Oncol, I-16148 Genoa, Italy Ist Giannina Gaslini Genoa Italy I-16148 Lab Oncol, I-16148 Genoa, Italy Natl Inst Canc Res, Genoa, Italy Natl Inst Canc Res Genoa ItalyNatl Inst Canc Res, Genoa, Italy Univ Genoa, Sect Human Anat, Dept Expt Med, Genoa, Italy Univ Genoa Genoa Italy oa, Sect Human Anat, Dept Expt Med, Genoa, Italy
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 2, volume: 253, anno: 1999,
pagine: 440 - 453
SICI:
0014-4827(199912)253:2<440:UAFSOT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN PERIPHERAL-BLOOD; LYMPHOCYTE MATURATION FACTOR; STIMULATORY FACTOR NKSF; VIRUS-INFECTED CELLS; REQUIRE GRANZYME-B; NATURAL-KILLER; T-CELLS; MEDIATED CYTOTOXICITY; CYTOLYTIC ACTIVITY; DNA FRAGMENTATION;
Keywords:
human NK cells; cytotoxicity; cytokines;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Facchetti, P Ist Giannina Gaslini, Lab Oncol, Largo G Gaslini 5, I-16148 Genoa, Italy Ist Giannina Gaslini Largo G Gaslini 5 Genoa Italy I-16148 y
Citazione:
P. Facchetti et al., "Ultrastructural and functional studies of the interaction between IL-12 and IL-2 for the generation of lymphokine-activated killer cells", EXP CELL RE, 253(2), 1999, pp. 440-453

Abstract

IL-12 promotes generation of LAT( activity in shortterm-cultured NI( cells, but information on the structure and function of IL-12-induced LAK cells is not yet available. The latter issues have been here investigated with emphasis on interactions between IL-12 and IL-2. Peripheral blood mononuclearcells (MNC) exposed to IL-12 for 5-7 days displayed a decrease in the amount and density of the matrix of large granular lymphocyte (LGL)-associated granules. In cells cultured with IL-12 and IL-2 for 5-7 days, empty vacuoles were predominant, and the electron-dense matrix was scanty. In MNC incubated with IL-2 for 5-7 days, most granules were loaded with electron-dense matrix. IL-12 and IL-2 displayed an additive effect on LAK cell cytotoxicityuntil approximately 48 h in culture which was followed by a sharp decline. Immunocytochemical and biochemical studies demonstrated that MNC cultured for 5-7 days with IL-12 and IL-2 displayed downregulated perforin expression and upregulated granzyme B expression. Pas ligand expression was virtually undetectable in MNC cultured for 5-7 days with or without cytokines. It appears that perforin downregulation plays a major role in the reduced cytotoxicity of MNC cultured with IL-12 and IL-2 for 5-7 days. (C) 1999 AcademicPress.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 07:43:30