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Titolo:
A RNA-POLYMERASE-III-BASED 2-HYBRID SYSTEM TO STUDY RNA-POLYMERASE-IITRANSCRIPTIONAL REGULATORS
Autore:
MARSOLIER MC; PRIOLEAU MN; SENTENAC A;
Indirizzi:
CEA SACLAY,SERV BIOCHEM & GENET MOL F-91191 GIF SUR YVETTE FRANCE CEA SACLAY,SERV BIOCHEM & GENET MOL F-91191 GIF SUR YVETTE FRANCE
Titolo Testata:
Journal of Molecular Biology
fascicolo: 2, volume: 268, anno: 1997,
pagine: 243 - 249
SICI:
0022-2836(1997)268:2<243:AR2STS>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-PROTEIN INTERACTIONS; SACCHAROMYCES-CEREVISIAE; SPLICING FACTORS; YEAST; GENE; PROMOTER; INITIATION; COMPLEX; DNA;
Keywords:
RNA POLYMERASE III; SACCHAROMYCES CEREVISIAE; SNR6; TRANSCRIPTIONAL ACTIVATORS; 2-HYBRID SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
M.C. Marsolier et al., "A RNA-POLYMERASE-III-BASED 2-HYBRID SYSTEM TO STUDY RNA-POLYMERASE-IITRANSCRIPTIONAL REGULATORS", Journal of Molecular Biology, 268(2), 1997, pp. 243-249

Abstract

In a previous study, we explored the mechanisms of SNR6 gene activation by grafting a heterologous DNA-binding domain, GAL4-(1-147), to various components of the yeast RNA polymerase III transcription system. Here, we demonstrate that a modified SNR6 gene harboring GAL4-binding sites (UAS(G)-SNR6) can be efficiently activated via an intervening, unrelated protein-protein interaction, thus laying the foundations of aRNA polymerase m-based two-hybrid system. In a model system, the interacting proteins recruiting TFIIIC to DNA were PRP21 and PRP9 or PRP21and PRP11. Mutations affecting the interaction between PRP21 and PRP9, or PRP21 and PRP11 decreased UAS(G)-SNR6 activation level proportionally. RNA polymerase II transcriptional activators, like GAL4, VP16 orp53, fused to GAL4 DNA-binding domain, did not activate the UAS(G)-SNR6 gene. However, GAL4 strongly activated UAS,(G)-SNR6 when GAL80, an interacting protein, was fused to TFIIIC. This result indicates that this two-hybrid system can be used to assess the interactions between RNA polymerase II regulatory proteins and their partners. (C) 1997 Academic Press Limited.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 03:33:10