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Titolo:
Correlation of positive RT-PCR for tyrosinase in peripheral blood of malignant melanoma patients with clinical stage, survival and other risk factors
Autore:
Proebstle, TM; Jiang, W; Hogel, J; Keilholz, U; Weber, L; Voit, C;
Indirizzi:
Humboldt Univ, Dept Dermatol, D-10117 Berlin, Germany Humboldt Univ Berlin Germany D-10117 t Dermatol, D-10117 Berlin, Germany Free Univ Berlin, Hosp Benjamin Franklin, Dept Med 3, Berlin, Germany FreeUniv Berlin Berlin Germany n Franklin, Dept Med 3, Berlin, Germany Univ Mainz, Dept Dermatol, D-6500 Mainz, Germany Univ Mainz Mainz Germany D-6500 nz, Dept Dermatol, D-6500 Mainz, Germany Univ Ulm, Dept Dermatol, Ulm, Germany Univ Ulm Ulm GermanyUniv Ulm, Dept Dermatol, Ulm, Germany Univ Ulm, Dept Biometry & Med Documentat, Ulm, Germany Univ Ulm Ulm Germany Ulm, Dept Biometry & Med Documentat, Ulm, Germany
Titolo Testata:
BRITISH JOURNAL OF CANCER
fascicolo: 1, volume: 82, anno: 2000,
pagine: 118 - 123
SICI:
0007-0920(200001)82:1<118:COPRFT>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; MESSENGER-RNA; TUMOR-CELLS; REVERSE-TRANSCRIPTASE; MARKER; SEQUENCE;
Keywords:
circulating tumour cells; minimal residual disease;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Voit, C Humboldt Univ, Dept Dermatol, Schumannstr 20-21, D-10117 Berlin, Germany Humboldt Univ Schumannstr 20-21 Berlin Germany D-10117 n, Germany
Citazione:
T.M. Proebstle et al., "Correlation of positive RT-PCR for tyrosinase in peripheral blood of malignant melanoma patients with clinical stage, survival and other risk factors", BR J CANC, 82(1), 2000, pp. 118-123

Abstract

The clinical value of the reverse transcription polymerase chain reaction (RT-PCR) assay for tyrosinase in peripheral blood of melanoma patients is still under debate. A total of 212 blood samples from 212 melanoma patients in all clinical stages (AJCC) were examined. Erythrocytes were lysed prior to RNA extraction by phenol precipitation from 2.7 mi of blood. cDNA for tyrosinase PCR was synthesized using random hexamers. Positive tyrosinase RT-PCR results were obtained in 11% of 106 stage I patients, 18% of 56 stage Ii patients, 31% of 26 stage III patients and 67% of 24 stage IV patients. After a median follow-up of 36 months (range 26-41), stage III patients withpositive RT-PCR for tyrosinase had a shortened disease-free interval as compared to negative patients (P < 0.01). In stage IV patients, median overall survival was 8 months in case of a positive RT-PCR in contrast to 12 months in case of a negative test. While univariate analysis showed sex and primary tumour location associated with positive RT-PCR, multiple regression analysis revealed clinical stage and detection of tyrosinase transcripts in peripheral blood as best prognostic factors. Hazard ratios for disease-freesurvival were 19.7 (confidence interval (CI) 8.53-45.5, P = 0.0001) for metastatic vs primary disease and 2.96 (CI 1.49-5.89, P = 0.002) for positivevs negative tyrosinase RT-PCR. The corresponding hazard ratios for overallsurvival were 97.0 (CI 12.7-741, P = 0.0001) and 4.33 (CI 1.69-11.1, P = 0.002). Our results emphasize the importance of tyrosinase RT-PCR testing inperipheral blood, (C) 2000 Cancer Research Campaign.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/01/20 alle ore 13:02:13