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Titolo:
Importance of nucleotide sequence and chemical modifications of antisense oligonucleotides
Autore:
Agrawal, S;
Indirizzi:
Hybridon Inc, Milford, MA 01757 USA Hybridon Inc Milford MA USA 01757Hybridon Inc, Milford, MA 01757 USA
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
fascicolo: 1, volume: 1489, anno: 1999,
pagine: 53 - 68
SICI:
0167-4781(199912)1489:1<53:IONSAC>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; MIXED-BACKBONE OLIGONUCLEOTIDES; IN-VIVO STABILITY; OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATE GEM-91; NUCLEOSIDE BICYCLIC OXAZAPHOSPHOLIDINES; PHASE STEREOSELECTIVE SYNTHESIS; CHRONICALLY INFECTED-CELLS; CPG-DNA; MESSENGER-RNA; TISSUE DISTRIBUTION;
Keywords:
antisense; oligonucleotide; mixed-backbone oligonucleotide; pharmacokinetics; immune stimulation; CpG; pro-drug; antiviral;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
111
Recensione:
Indirizzi per estratti:
Indirizzo: Agrawal, S Hybridon Inc, 155 Fortune Blvd, Milford, MA 01757 USA Hybridon Inc 155 Fortune Blvd Milford MA USA 01757 MA 01757 USA
Citazione:
S. Agrawal, "Importance of nucleotide sequence and chemical modifications of antisense oligonucleotides", BBA-GENE ST, 1489(1), 1999, pp. 53-68

Abstract

The antisense approach is conceptually simple and elegant; to design an inhibitor of a specific mRNA, one needs only to know the sequence of the targeted mRNA and an appropriately modified complementary oligonucleotide. Of the many analogs of oligodeoxynucleotides explored as antisense agents, phosphorothioate analogs have been studied the most extensively. The use of phosphorothioate oligodeoxynucleotides as antisense agents in various studies have shown promising results. However, they have also indicated that quite often, biological effects observed could be solely or partly non-specific in nature. It is becoming clear that not all phosphorothioate oligodeoxynucleotides of varying length and base composition are the same, and important consideration should be given to maintain antisense mechanisms while identifying effective antisense oligonucleotides. In this review, I have summarized the progress made in my laboratory in understanding the specificity and mechanism of actions of phosphorothioate oligonucleotides and the rationalefor designing second-generation mixed-backbone oligonucleotides. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 00:08:54