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Titolo:
Chemiluminescence determination of catecholamines in human blood plasma using 1,2-bis(3-chlorophenyl)ethylenediamine as pre-column derivatizing reagent for liquid chromatography
Autore:
Ragab, GH; Nohta, H; Zaitsu, K;
Indirizzi:
Kyushu Univ, Grad Sch Pharmaceut Sci, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 Pharmaceut Sci, Fukuoka 8128582, Japan
Titolo Testata:
ANALYTICA CHIMICA ACTA
fascicolo: 1-2, volume: 403, anno: 2000,
pagine: 155 - 160
SICI:
0003-2670(20000103)403:1-2<155:CDOCIH>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTROCHEMICAL DETECTION; PRECOLUMN DERIVATIZATION; CHEMI-LUMINESCENCE; 1,2-DIPHENYLETHYLENEDIAMINE; NOREPINEPHRINE; DOPAMINE; HPLC; EPINEPHRINE; SYSTEM; ASSAY;
Keywords:
catecholamine; liquid chromatography; peroxyoxalate; chemiluminescence; norepinephrine; epinephrine; dopamine; 1,2-bis(3-chlorophenyl)ethylene diamine; human blood plasma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Zaitsu, K Kyushu Univ, Grad Sch Pharmaceut Sci, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 Sci, Fukuoka 8128582, Japan
Citazione:
G.H. Ragab et al., "Chemiluminescence determination of catecholamines in human blood plasma using 1,2-bis(3-chlorophenyl)ethylenediamine as pre-column derivatizing reagent for liquid chromatography", ANALYT CHIM, 403(1-2), 2000, pp. 155-160

Abstract

A highly sensitive method for the determination of the human blood plasma catecholamines (norepinephrine, epinephrine and dopamine) is described which employs liquid chromatography (LC) with chemiluminescence detection. Solid-phase extraction using cation exchange cartridges was used for selective and quantitative isolation of the catecholamines and isoproterenol tan internal standard) from 20 mu l of human plasma containing 0.1 pmol ml(-1) isoproterenol. The amines and isoproterenol, derivatized with 1,2-bis(3-chlorophenyl)ethylene diamine, were separated on a reversed-phase LC column with isocratic elution using a mixture of imidazole buffer (120 mM, pH 5.8), methanol, acetonitrile (13:4: 18, v/v/v). The eluate was detected by a post-column chemiluminescence reaction system using bis[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl) phenyl]oxalate and hydrogen peroxide. The detection limits (asignal-to-noise ratio = 3) for each catecholamine are 40-120 amol in a 100mu l injection volume. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:10:09