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Titolo:
An automated biological assay to determine levels of the trypanocidal drugmelarsoprol in biological fluids
Autore:
Onyango, JD; Burri, C; Brun, R;
Indirizzi:
Swiss Trop Inst, CH-4002 Basel, Switzerland Swiss Trop Inst Basel Switzerland CH-4002 st, CH-4002 Basel, Switzerland Kenya Trypanosomiasis Res Inst, Kikuyu, Kenya Kenya Trypanosomiasis Res Inst Kikuyu Kenya sis Res Inst, Kikuyu, Kenya
Titolo Testata:
ACTA TROPICA
fascicolo: 1, volume: 74, anno: 2000,
pagine: 95 - 100
SICI:
0001-706X(20000105)74:1<95:AABATD>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRYPANOSOMA-BRUCEI-RHODESIENSE; GAMBIENSE SLEEPING SICKNESS; T-GAMBIENSE; IN-VITRO; CULTIVATION; SENSITIVITY; RESISTANCE; BIOASSAY;
Keywords:
fluorometry; Trypanosoma brucei; Alamar blue; bioassay; melarsoprol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Burri, C Swiss Trop Inst, Socinstr 57, CH-4002 Basel, Switzerland Swiss Trop Inst Socinstr 57 Basel Switzerland CH-4002 witzerland
Citazione:
J.D. Onyango et al., "An automated biological assay to determine levels of the trypanocidal drugmelarsoprol in biological fluids", ACT TROP, 74(1), 2000, pp. 95-100

Abstract

For the investigation of the pharmacokinetic properties of a drug, methodsfor sensitive and precise quantification are a prerequisite. Only few functional methods exist for the determination of the trypanocidal drug melarsoprol in biological fluids: A bioassay which requires microscopical evaluation and two HPLC methods, which require sample extraction and are difficult to automatize due to the drug's properties. We report the development of anautomated biological assay, based on the fluorescent dye Alamar blue(R). To validate the assay for melarsoprol, 108 serum and 37 cerebrospinal fluid (CSF) samples were spiked with melarsoprol at concentrations of 17-92 ng/mlfor CSF and 17 ng/ml-2.2 mu g/ml for serum. The precision (repeatability) expressed as the interday average coefficient of variation was 9.9% for serum and 18.8% for CSF samples over the respective concentration range. The accuracy (measurement for the systematic error) of the test was 99.4% for serum and 96.4% for CSF. The assay's limit of quantitation with the use of the trypanosome stock STI 704 BABA was 4 ng/ml for both serum and CSF samples. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 10:04:40