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Titolo:
Increased levels of tissue factor mRNA in mononuclear blood cells of patients with primary antiphospholipid syndrome
Autore:
Dobado-Berrios, PM; Lopez-Pedrera, C; Velasco, F; Aguirre, MA; Torres, A; Cuadrado, MJ;
Indirizzi:
Univ Cordoba, Hosp Reina Sofia, Unidad Invest, Dept Res, E-14004 Cordoba, Spain Univ Cordoba Cordoba Spain E-14004 est, Dept Res, E-14004 Cordoba, Spain Univ Cordoba, Hosp Reina Sofia, Dept Haematol & Haemotherapy, E-14004 Cordoba, Spain Univ Cordoba Cordoba Spain E-14004 Haemotherapy, E-14004 Cordoba, Spain Univ Cordoba, Hosp Reina Sofia, Dept Rheumatol, E-14004 Cordoba, Spain Univ Cordoba Cordoba Spain E-14004 ept Rheumatol, E-14004 Cordoba, Spain
Titolo Testata:
THROMBOSIS AND HAEMOSTASIS
fascicolo: 6, volume: 82, anno: 1999,
pagine: 1578 - 1582
SICI:
0340-6245(199912)82:6<1578:ILOTFM>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCYTE PROCOAGULANT ACTIVITY; SYSTEMIC LUPUS-ERYTHEMATOSUS; ANTIBODY-MEDIATED THROMBOSIS; CULTURED ENDOTHELIAL-CELLS; TUMOR-NECROSIS-FACTOR; FACTOR EXPRESSION; ANTICARDIOLIPIN ANTIBODIES; HYPERCOAGULABLE STATE; INDUCTION; ADHERENCE;
Keywords:
antiphospholipid syndrome; in vivo; monocyte; mRNA; tissue factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Dobado-Berrios, PM Univ Cordoba, Hosp Reina Sofia, Unidad Invest, Dept Res, Avda Menendez Pidal S-N, E-14004 Cordoba, Spain Univ Cordoba Avda Menendez Pidal S-N Cordoba Spain E-14004
Citazione:
P.M. Dobado-Berrios et al., "Increased levels of tissue factor mRNA in mononuclear blood cells of patients with primary antiphospholipid syndrome", THROMB HAEM, 82(6), 1999, pp. 1578-1582

Abstract

Antiphospholipid antibodies (aPL) may stimulate tissue factor (TF) expression in cultured endothelial cells and monocytes, but there are discrepancies as to the expression of TF in the patients with antiphospholipid syndrome(APS). By using reverse transcription and polymerase chain reaction amplification, we have analysed TF mRNA accumulation in freshly isolated mononuclear blood cells (MBC)of 14 patients with primary APS (PAPS) and six normal controls. TF mRNA accumulation was low or absent in uncultured MBC from allnormal controls, but was elevated in uncultured MBC from nine of the patients as well as in normal MBC incubated with 100 ng/ml lipopolysaccharide (LPS). Mean levels of TF mRNA, as measured by densitometry, were higher in MBC from patients (N = 14) than in those from controls (N = 6, P = 0.009), and in MBC from patients with a history of thrombosis (N = 9) than in those from patients without thrombosis (N = 5, P = 0.02). Uncultured MBC of patients with thrombosis accumulated TF mRNA at similar levels to LPS-treated normal MBC. Increased levels of TF mRNA were found in eight of ten patients with conventional aPL (ie, anti-cardiolipin antibodies [aCL] and/or lupus anticoagulant [LA]) and little if any accumulation of TF mRNA was observed in three of four patients without aPL at the time of study. These data strongly suggest that circulating monocytes of many patients with PAPS are subjected to an up-regulated TF expression that may well explain their prothrombotic state. Although the presence or absence of TF mRNA in MBC was associatedwith, respectively, the presence or absence of conventional aPL in 11 of the 14 patients studied, our study cannot exclude the involvement of factorsother than aCL or LA in inducing TF expression.

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Documento generato il 11/07/20 alle ore 20:02:18