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Titolo:
Characteristics of human T-lymphotropic virus type-1 (HTLV-1)-infected cell line MT-2, which is not killed by a natural killer cell line NK-92 but iskilled by lymphokine-activated killer cells
Autore:
Komatsu, F; Yoshida, S;
Indirizzi:
Tokyo Med & Dent Univ, Sch Med, Blood Transfus Serv, Bunkyo Ku, Tokyo 1138519, Japan Tokyo Med & Dent Univ Tokyo Japan 1138519 unkyo Ku, Tokyo 1138519, Japan
Titolo Testata:
ONCOLOGY RESEARCH
fascicolo: 5, volume: 11, anno: 1999,
pagine: 213 - 218
SICI:
0965-0407(1999)11:5<213:COHTVT>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLASS-I; TARGET-CELLS; EXPRESSION; RECEPTOR; LEUKEMIA;
Keywords:
MT-2; NK-92; lymphokine-activated killer (LAK); human T-lymphotropic virus type 1 (HTLV-1); natural killer cell inhibitory receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Komatsu, F Tokyo Med & Dent Univ, Sch Med, Blood Transfus Serv, Bunkyo Ku,Yushima 1-5-45, Tokyo 1138519, Japan Tokyo Med & Dent Univ Yushima 1-5-45 Tokyo Japan 1138519 Japan
Citazione:
F. Komatsu e S. Yoshida, "Characteristics of human T-lymphotropic virus type-1 (HTLV-1)-infected cell line MT-2, which is not killed by a natural killer cell line NK-92 but iskilled by lymphokine-activated killer cells", ONCOL RES, 11(5), 1999, pp. 213-218

Abstract

Natural killer (NK) cell-mediated cytolysis (NK-lysis) is triggered by costimulatory signals of adhesion molecules and is downregulated by negative signals of killer cell inhibitory receptors (KIRs). Recently, a NK cell line, NK-92, was established. This cell line can kill several tumor cells, which possess adhesion molecules CD54 and CD102. However, the NK-92 cannot killa human T-lymphotropic virus type 1 (HTLV-l)-infected cell line, MT-2, although lymphokine-activated killer (LAK) cells can kill MT-2. In this reportwe investigated the reason for LAK sensitivity but NK-92 resistance of theMT-2. The MT-2 highly expressed CD54 and CD102, suggesting that the costimulatory signals may be intact. Then we tested the responsibility of the negative signals by determining HLA type of the MT-2 and KIRs of the effector cells. The MT-2 expressed HLA-A24, B40, B51, Cw3, and HLA-G. The NK-92 did not express KIR2DL1, KIR2DL2,3, nor KIR3DL1, but 24% of the cells weakly expressed CD94. The blocking tests against these HLA class I molecules and Kms did not restore the NK-92 resistance, although blocking against HLA-G slightly increased its lysis. Finally, in order to eliminate the class I molecules from the cell surface, we treated the MT-2 using a buffered citric acid solution (pH 3.8). By using this treatment, the expression of class I molecules and HTLV-1 antigen decreased, and then the MT-2 was killed by the NK-92. These findings suggest that an aberrant class I molecule of the MT-2 transferred a negative signal to the NK-92 and induced the NK-92 resistance.rt remains to be elucidated whether or not the HTLV-l infection contributed to the alteration of the class I molecule.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 18:58:02