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Titolo:
A structural change in the kinesin motor protein that drives motility
Autore:
Rice, S; Lin, AW; Safer, D; Hart, CL; Naber, N; Carragher, BO; Cain, SM; Pechatnikova, E; Wilson-Kubalek, EM; Whittaker, M; Pate, E; Cooke, R; Taylor, EW; Milligan, RA; Vale, RD;
Indirizzi:
Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Dept Mol & Cellular Pharmacol, San Francisco, CA94143 USA Univ Calif San Francisco San Francisco CA USA 94143 rancisco, CA94143 USA Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Scripps Clin & Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA Scripps Clin & Res Inst La Jolla CA USA 92037 iol, La Jolla, CA 92037 USA Univ Penn, Sch Med, Dept Physiol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 t Physiol, Philadelphia, PA 19104 USA Univ Illinois, Beckman Inst, Dept Cell & Struct Biol, Urbana, IL 61801 USAUniv Illinois Urbana IL USA 61801 ell & Struct Biol, Urbana, IL 61801 USA Univ Chicago, Dept Mol Genet & Cell Biol, Chicago, IL 60637 USA Univ Chicago Chicago IL USA 60637 enet & Cell Biol, Chicago, IL 60637 USA Washington State Univ, Dept Pure & Appl Math, Pullman, WA 99164 USA Washington State Univ Pullman WA USA 99164 pl Math, Pullman, WA 99164 USA
Titolo Testata:
NATURE
fascicolo: 6763, volume: 402, anno: 1999,
pagine: 778 - 784
SICI:
0028-0836(199912)402:6763<778:ASCITK>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRYSTAL-STRUCTURE; DIMERIC KINESIN; MYOSIN MOTOR; CRYOELECTRON MICROSCOPY; ATP HYDROLYSIS; MICROTUBULE; DOMAIN; MECHANISM; COMPLEX; NCD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Vale, RD Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 CA 94143 USA
Citazione:
S. Rice et al., "A structural change in the kinesin motor protein that drives motility", NATURE, 402(6763), 1999, pp. 778-784

Abstract

Kinesin motors power many motile processes by converting ATP energy into unidirectional motion along microtubules, The force-generating and enzymaticproperties of conventional kinesin have been extensively studied; however,the structural basis of movement is unknown. Here we have detected and visualized a large conformational change of a similar to 15-amino-acid region (the neck linker) in kinesin using electron paramagnetic resonance, fluorescence resonance energy transfer, pre-steady state kinetics and cryo-electron microscopy, This region becomes immobilized and extended towards the microtubule 'plus' end when kinesin binds microtubules and ATP, and reverts to a more mobile conformation when gamma-phosphate is released after nucleotide hydrolysis, This conformational change explains both the direction of kinesin motion and processive movement by the kinesin dimer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 06:07:01