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Titolo:
Nuclear accumulation of S-adenosylhomocysteine hydrolase in transcriptionally active cells during development of Xenopus laevis
Autore:
Radomski, N; Kaufmann, C; Dreyer, C;
Indirizzi:
Max Planck Inst Entwicklungsbiol, D-72076 Tubingen, Germany Max Planck Inst Entwicklungsbiol Tubingen Germany D-72076 ingen, Germany
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 12, volume: 10, anno: 1999,
pagine: 4283 - 4298
SICI:
1059-1524(199912)10:12<4283:NAOSHI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA-POLYMERASE-II; DNA METHYLATION; PROTEINS; EMBRYOS; EXPRESSION; BINDING; ADENOSINE; DOMAINS; FATE; PHOSPHORYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Dreyer, C Max Planck Inst Entwicklungsbiol, D-72076 Tubingen, Germany Max Planck Inst Entwicklungsbiol Tubingen Germany D-72076 many
Citazione:
N. Radomski et al., "Nuclear accumulation of S-adenosylhomocysteine hydrolase in transcriptionally active cells during development of Xenopus laevis", MOL BIOL CE, 10(12), 1999, pp. 4283-4298

Abstract

The oocyte nuclear antigen of the monoclonal antibody 32-5B6 of Xenopus laevis is subject to regulated nuclear translocation during embryogenesis. Itis distributed in the cytoplasm during oocyte maturation, where it remainsduring cleavage and: blastula stages, before it gradually reaccumulates inthe nuclei during gastrulation, We have now identified this antigen to be the enzyme S-adenosylhomocysteine hydrolase (SAHH). SAHH is the only enzymethat cleaves S-adenosylhomocysteine, a reaction product and an inhibitor of all S-adenosylmethionine-dependent methylation reactions. We have compared the spatial and temporal patterns of nuclear localization of SAHH and of nuclear methyltransferase activities during embryogenesis and in tissue culture cells. Nuclear localization of Xenopus SAHH did not temporally correlate with DNA methylation. However, we found that SAHH nuclear localization coincides with high rates of mRNA synthesis, a subpopulation colocalizes With RNA polymerase II, and inhibitors of SAHH reduce both methylation and synthesis of poly(A)(+) RNA. We therefore propose that accumulation of SAHH inthe nucleus may be required for efficient cap methylation in transcriptionally active cells. Mutation analysis revealed that the C terminus and the Nterminus are both required for efficient nuclear translocation in tissue culture cells, indicating that more than one interacting domain contributes to nuclear accumulation of Xenopus SAHH.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/08/20 alle ore 20:14:14