Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Regulation of prohormone convertase 1 (PC1) by gp130-related-cytokines
Autore:
Li, QL; Jansen, E; Friedman, TC;
Indirizzi:
Univ Calif Los Angeles, Sch Med, Cedars Sinai Res Inst, Dept Med,Div Endocrinol, Los Angeles, CA 90048 USA Univ Calif Los Angeles Los Angeles CA USA90048 Los Angeles, CA 90048 USA Katholieke Univ Leuven, Ctr Human Genet, Oncol Mol Lab, Louvain, Belgium Katholieke Univ Leuven Louvain Belgium Oncol Mol Lab, Louvain, Belgium Flanders Interuniv Inst Biotechnol, B-3000 Louvain, Belgium Flanders Interuniv Inst Biotechnol Louvain Belgium B-3000 uvain, Belgium
Titolo Testata:
MOLECULAR AND CELLULAR ENDOCRINOLOGY
fascicolo: 1-2, volume: 158, anno: 1999,
pagine: 143 - 152
SICI:
0303-7207(199912)158:1-2<143:ROPC1(>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEUKEMIA INHIBITORY FACTOR; PROOPIOMELANOCORTIN GENE-EXPRESSION; COMPLEMENTARY DEOXYRIBONUCLEIC-ACID; KEX2-LIKE PROCESSING ENDOPROTEASE; CORTICOTROPIN-RELEASING HORMONE; PITUITARY TUMOR-CELLS; PROPROTEIN CONVERTASE; MESSENGER-RNA; ADRENOCORTICOTROPIC HORMONE; ATT-20 CELLS;
Keywords:
IL-6; LIF; peptide biosynthesis; processing enzymes; post-translational processing; POMC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Friedman, TC Univ Calif Los Angeles, Sch Med, Cedars Sinai Res Inst, Dept Med,Div Endocrinol, Los Angeles, CA 90048 USA Univ Calif Los Angeles Los Angeles CA USA 90048 CA 90048 USA
Citazione:
Q.L. Li et al., "Regulation of prohormone convertase 1 (PC1) by gp130-related-cytokines", MOL C ENDOC, 158(1-2), 1999, pp. 143-152

Abstract

The processing of pro-opiomelanocortin (POMC) to generate bioactive ACTH in the anterior pituitary is mediated by prohormone convertase 1 (PC1). Leukemia inhibitory factor (LIF) and interleukin 6 (IL-6), two cytokines sharing the common gp130 receptor subunit and functioning through activation of the intracellular JAK/STAT pathway, induce POMC synthesis and ACTH release. We investigated the effects of LIF and IL-6 on PC1 expression and its subsequent processing of POMC. A significant time-dependent up-regulation of both PC1 protein and mRNA by LIF and IL-6 was seen in mouse corticotroph AtT-20 cells. IL-6 or LIF increased the synthesis of ACTH-related products with a concomitant increase in bioactive 5 and 13 kDa ACTH indicating coordinated regulation of substrate and processing enzyme. AtT-20 cells transiently transfected with a human PC1-promoter-luciferase reporter construct and treated with LIF or IL-6 showed significantly increased luciferase activity. Additionally, lipopolysaccharide (LPS) administration to rats resulted in an increase in both pituitary PC1 and POMC mRNA. These findings suggest that the ACTH increase induced by LIF and IL-6 is due to both increased POMC synthesis as well as increased POMC processing by up-regulation of PC1. These two coordinately regulated processing events probably exert central roles inthe pathophysiological response to some stresses, such as inflammatory stress. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 13:22:33