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Titolo:
Activated mutants of SHP-2 preferentially induce elongation of Xenopus animal caps
Autore:
OReilly, AM; Pluskey, S; Shoelson, SE; Neel, BG;
Indirizzi:
Beth Israel Deaconess Med Ctr, Dept Med, Div Hematol Oncol, Canc Biol Program, Boston, MA 02215 USA Beth Israel Deaconess Med Ctr Boston MA USA 02215 m, Boston, MA 02215 USA Harvard Univ, Sch Med, Brigham & Womens Hosp, Joslin Diabet Ctr, Boston, MA USA Harvard Univ Boston MA USA omens Hosp, Joslin Diabet Ctr, Boston, MA USA Harvard Univ, Sch Med, Brigham & Womens Hosp, Dept Med, Boston, MA USA Harvard Univ Boston MA USA igham & Womens Hosp, Dept Med, Boston, MA USA
Titolo Testata:
MOLECULAR AND CELLULAR BIOLOGY
fascicolo: 1, volume: 20, anno: 2000,
pagine: 299 - 311
SICI:
0270-7306(200001)20:1<299:AMOSPI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-TYROSINE-PHOSPHATASE; DOMINANT-NEGATIVE MUTANT; CELL-SHAPE CHANGES; MESODERM INDUCTION; MAP KINASE; GASTRULATION MOVEMENTS; SIGNAL-TRANSDUCTION; TERMINAL SEQUENCE; C-CADHERIN; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Neel, BG Beth Israel Deaconess Med Ctr, Dept Med, Div Hematol Oncol, Canc Biol Program, HIM 1047,330 Brookline Ave, Boston, MA 02215 USA Beth Israel Deaconess Med Ctr HIM 1047,330 Brookline Ave Boston MA USA 02215
Citazione:
A.M. O'Reilly et al., "Activated mutants of SHP-2 preferentially induce elongation of Xenopus animal caps", MOL CELL B, 20(1), 2000, pp. 299-311

Abstract

In Xenopus ectodermal explants (animal caps), fibroblast growth factor (FGF) evokes two major events: induction of ventrolateral mesodermal tissues and elongation. The Xenopus FGF receptor (XFGFR) and certain downstream components of the XFGFR signal transduction pathway (e.g., members of the Ras/Raf/MEK/mitogen-activated protein kinase [MAPK] cascade) are required for both of these processes. Likewise, activated versions of these signaling components induce mesoderm and promote animal cap elongation. Previously, usinga dominant negative mutant approach, we showed that the protein-tyrosine phosphatase SHP-2 is necessary for FGF-induced MAPK activation, mesoderm induction, and elongation of animal caps. Taking advantage of recent structural information, we now have generated novel, activated mutants of SHP-2. Here, we show that expression of these mutants induces animal cap elongation to an extent comparable to that evoked by FGF. Surprisingly, however, activated mutant-induced elongation can occur without mesodermal cytodifferentiation and is accompanied by minimal activation of the MAPK pathway and mesodermal marker expression. Our results implicate SHP-2 in a pathway(s) directing cell movements in vivo and identify potential downstream components of this pathway. Our activated mutants also may be useful for determining the specific functions of SHP-2 in other signaling systems.

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Documento generato il 23/01/21 alle ore 03:02:46