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Titolo:
Competition between Li+ and Mg2+ for red blood cell membrane phospholipids: A P-31, Li-7, and Li-6 nuclear magnetic resonance study
Autore:
Srinivasan, C; Minadeo, N; Geraldes, CFGC; de Freitas, DM;
Indirizzi:
Loyola Univ, Dept Chem, Chicago, IL 60626 USA Loyola Univ Chicago IL USA 60626 a Univ, Dept Chem, Chicago, IL 60626 USA Univ Coimbra, Dept Biochem, P-3000 Coimbra, Portugal Univ Coimbra Coimbra Portugal P-3000 t Biochem, P-3000 Coimbra, Portugal Univ Coimbra, Ctr Neurosci, P-3000 Coimbra, Portugal Univ Coimbra Coimbra Portugal P-3000 Neurosci, P-3000 Coimbra, Portugal
Titolo Testata:
LIPIDS
fascicolo: 11, volume: 34, anno: 1999,
pagine: 1211 - 1221
SICI:
0024-4201(199911)34:11<1211:CBLAMF>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ERYTHROCYTE-MEMBRANE; AQUEOUS-SOLUTION; MAGNESIUM-IONS; LITHIUM ION; NMR; TRANSPORT; BINDING; NA+; ATP; SPECTROSCOPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: de Freitas, DM Loyola Univ, Dept Chem, 6525 N Sheridan Rd, Chicago, IL 60626 USA Loyola Univ 6525 N Sheridan Rd Chicago IL USA 60626 626 USA
Citazione:
C. Srinivasan et al., "Competition between Li+ and Mg2+ for red blood cell membrane phospholipids: A P-31, Li-7, and Li-6 nuclear magnetic resonance study", LIPIDS, 34(11), 1999, pp. 1211-1221

Abstract

The mode of action of the lithium ion (Li+) in the treatment of manic depression or bipolar illness is still under investigation, although this inorganic drug has been in clinical use for 50 yr. Several research reports haveprovided evidence for Li+/Mg2+ competition in biomolecules. We carried outthis study to characterize the interactions of Li+ and Mg2+ with red bloodcell (RBC) membrane components to see whether Li+/Mg2+ competition occurs. P-31 nuclear magnetic resonance chemical shift measurements of the phospholipids extracted from the RBC membranes indicated that the anionic phospho-lipids phosphatidylserine and phosphatidylinositol, bind Li+ and Mg2+ most strongly . From Li-6 relaxation measurements, the Li+ binding constant to the phospholipid extract was found to be 45 +/- 5 M-1. Thus, these studies showed that the phospholipids play a major role in metal ion binding. Li-7 spin-lattice relaxation measurements conducted on unsealed and cytoskeleton-depleted RBC membrane in the presence of magnesium indicated that the removal of the cytoskeleton increases lithium binding to the more exposed anionic phospholipids (357 +/- 24 M-1) when compared to lithium binding in the unsealed RBC membrane (221 +/- 21 M-1). Therefore, it can be seen that the cytoskeleton does not play a major role in Li+ binding or in Li+/Mg2+ competition.

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Documento generato il 02/12/20 alle ore 05:33:31