Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Transplantation of highly purified peripheral blood CD34(+) cells from HLA-mismatched parental donors in 14 children: evaluation of early monitoring of engraftment
Autore:
Peters, C; Matthes-Martin, S; Fritsch, G; Holter, W; Lion, T; Witt, V; Hocker, P; Fischer, G; Dieckmann, K; Handgretinger, R; Klingebiel, T; Gadner, H;
Indirizzi:
St Anna Childrens Hosp, Childrens Canc Res Inst, A-1090 Vienna, Austria StAnna Childrens Hosp Vienna Austria A-1090 nst, A-1090 Vienna, Austria Univ Clin Vienna, Inst Blood Grp Serol & Transfus Med, Vienna, Austria Univ Clin Vienna Vienna Austria p Serol & Transfus Med, Vienna, Austria Univ Clin Vienna, Inst Radiotherapy, Vienna, Austria Univ Clin Vienna Vienna Austria nna, Inst Radiotherapy, Vienna, Austria Univ Clin Tuebingen, Dept Pediat, Tubingen, Germany Univ Clin Tuebingen Tubingen Germany en, Dept Pediat, Tubingen, Germany
Titolo Testata:
LEUKEMIA
fascicolo: 12, volume: 13, anno: 1999,
pagine: 2070 - 2078
SICI:
0887-6924(199912)13:12<2070:TOHPPB>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-MARROW TRANSPLANTATION; COLONY-STIMULATING FACTOR; ACUTE LYMPHOBLASTIC-LEUKEMIA; CHRONIC MYELOID-LEUKEMIA; VERSUS-HOST DISEASE; STEM-CELLS; MYELOGENOUS LEUKEMIA; PROGENITOR CELLS; GRAFT FAILURE; ALLOGRAFTS;
Keywords:
PBSCT; mismatched; magnetic beads; engraftment; chimerism; rejection;
Tipo documento:
Editorial Material
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Peters, C St Anna Childrens Hosp, Childrens Canc Res Inst, Kinderspitalgasse 6, A-1090 Vienna, Austria St Anna Childrens Hosp Kinderspitalgasse 6 Vienna Austria A-1090
Citazione:
C. Peters et al., "Transplantation of highly purified peripheral blood CD34(+) cells from HLA-mismatched parental donors in 14 children: evaluation of early monitoring of engraftment", LEUKEMIA, 13(12), 1999, pp. 2070-2078

Abstract

HLA-mismatched family members may represent an important cell source for patients that require stem cell transplantation but lack both a matched sibling donor and a closely matched unrelated donor. We report the outcome of 19 transplantations from HLA two- or three- loci mismatched parental donors in which 14 pediatric patients with hematological malignancies or other disorders, received a median of 21.5 x 10(6) (range, 5.4-58) highly purified CD34(+) peripheral blood stem cells (PBSC), as well as 4.7 x 10(4) (range, 0.4-12) donor T cells per kg body weight. T cell depletion was performed using a two-step CD34-positive selection on two different magnetic beads devices. Ten of 14 patients presented with rapid myeloid engraftment. The four patients who presented with graft failure (two non-engraftments, two rejections) received a second stem cell graft and one a third. Graft rejection wasdetected early by polymerase chain reaction (PCR) analysis of FAGS-sorted T cells. Eight of the 14 patients are still alive after a median observation period of 15.6 months (range, 3-31.3) with full donor chimerism in all hematopoietic cell lineages. No acute organ graft-versus-host disease (GVHD) and no chronic GVHD have occurred. One patient experienced relapse of leukemia. We conclude that transplantation of allogeneic PBSC from haploidentical donors will open new perspectives for pediatric patients far whom an HLA-matched stem cell graft is not available. Close monitoring of recipient anddonor hematopoiesis might be of clinical value, to recognize early engraftment or rejection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/10/20 alle ore 20:57:41