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Titolo:
The disappearance of cyclins A and B and the increase in activity of the G(2)/M-phase cellular kinase cdc2 in herpes simplex virus 1-infected cells require expression of the alpha 22/U(S)1.5 and U(L)13 viral genes
Autore:
Advani, SJ; Brandimarti, R; Weichselbaum, RR; Roizman, B;
Indirizzi:
Univ Chicago, Marjorie B Kovler Viral Oncol Labs, Chicago, IL 60637 USA Univ Chicago Chicago IL USA 60637 Viral Oncol Labs, Chicago, IL 60637 USA Univ Chicago, Dept Radiat & Cellular Oncol, Chicago, IL 60637 USA Univ Chicago Chicago IL USA 60637 & Cellular Oncol, Chicago, IL 60637 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 1, volume: 74, anno: 2000,
pagine: 8 - 15
SICI:
0022-538X(200001)74:1<8:TDOCAA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE; TYROSINE KINASE; INFECTED-CELLS; HUMAN WEE1; M-PHASE; PHOSPHORYLATION; ACTIVATION; HERPES-SIMPLEX-VIRUS-1; PHOSPHATASE; REPLICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Roizman, B Univ Chicago, Marjorie B Kovler Viral Oncol Labs, 910 E 58Th St, Chicago, IL 60637 USA Univ Chicago 910 E 58Th St Chicago IL USA 60637 o, IL 60637 USA
Citazione:
S.J. Advani et al., "The disappearance of cyclins A and B and the increase in activity of the G(2)/M-phase cellular kinase cdc2 in herpes simplex virus 1-infected cells require expression of the alpha 22/U(S)1.5 and U(L)13 viral genes", J VIROLOGY, 74(1), 2000, pp. 8-15

Abstract

In uninfected cells the G(2)/M transition is regulated by cyclin kinase complex containing cdc2 and, initially, cyclin A, followed by cyclin Il, cdc2is downregulated through phosphorylation by wee-1 and myt-1 and upregulated by cdc-25C phosphatase. We have examined the accumulation and activities of these proteins in cells infected with wild type and mutants of herpes simplex virus 1, The results were as follows. (i) Cyclin A and B levels were reduced beginning 4 h after infection and were undetectable at 12 to 16 h after infection. (ii) cdc2 protein also decreased in amount but was detectable at all times after infection. In addition, a fraction of cdc2 protein from infected cells exhibited altered electrophoretic mobility in denaturing gels. (iii) The Levels of cdk7 or myt-1 proteins remained relatively constant throughout infection, whereas the level of wee-1 was significantly decreased, (iv) cdc-25C formed novel bands characterized by slower electrophoretic mobility that disappeared after treatment with phosphatase, In addition,one phosphatase-sensitive band reacted with MPM-2 antibody that recognizesa phosphoepitope phosphorylated exclusively in M phase. (v) cdc2 accumulating in infected cells exhibited kinase activity. The activity of cdc2 was higher in infected cell lysates than those of corresponding proteins presentin lysates of mock-infected cells even though cyclins A and B were not detectable in lysates of infected cells, (vi) The decrease in the levels of cyclins A and B, the increase in activity of cdc2, and the hyperphosphorylation of cdc-25C were mediated by U(L)13 and alpha 22/U(S)1.5 gene products, In light of its normal functions, the activated cdc2 kinase may play a role in the changes in the morphology of the infected cell, These results are consistent with the accruing evidence that herpes simplex virus scavenges thecell for useful cell cycle proteins and subverts them for its own use.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 00:51:06