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Titolo:
Evidence for an inactive transposable mariner-like element in Anopheles albimanus
Autore:
Liu, KY; Lizardi, P; Yu, JZ; Rodriguez, MH;
Indirizzi:
Natl Autonomous Univ Mexico, Inst Biotechnol, Cuernavaca 62271, Morelos, Mexico Natl Autonomous Univ Mexico Cuernavaca Morelos Mexico 62271 relos, Mexico Inst Nacl Salud Publ, Cuernavaca 62508, Morelos, Mexico Inst Nacl Salud Publ Cuernavaca Morelos Mexico 62508 508, Morelos, Mexico
Titolo Testata:
JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION
fascicolo: 4, volume: 15, anno: 1999,
pagine: 463 - 467
SICI:
8756-971X(199912)15:4<463:EFAITM>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENETIC-TRANSFORMATION; DROSOPHILA; DNA;
Keywords:
transposon; mariner; Anopheles albimanus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Liu, KY Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Ave IPN 2508,Mexico City 07360, DF, Mexico Inst Politecn Nacl Ave IPN 2508 Mexico City DF Mexico 07360 Mexico
Citazione:
K.Y. Liu et al., "Evidence for an inactive transposable mariner-like element in Anopheles albimanus", J AM MOSQ C, 15(4), 1999, pp. 463-467

Abstract

Transposable elements may eventually be used as transformation Vectors of anti-Plasmodium genes in natural populations of Anopheles mosquitoes. The mariner transposable element is widespread in most animal phyla and we wished to test for its presence in the important Latin American malaria vector, Anogheles albimanus. Degenerate primers were designed from the consensus ofinsect mariner elements and used in a polymerase chain reaction to amplifya fragment of the predicted size from An. albimanus. The fragment was cloned, sequenced, and determined to be a mariner-like element through multiplealignment with known insect mariner elements. Dot blot and Southern blot analyses showed only one or a few elements per haploid genome. The cloned fragment was used as a probe to isolate similar sequences from an An. albimanus genomic DNA library. A 659-base-pair done was 57% similar at the DNA level with mariner elements from Anopheles gambiae, Chrysoperla ptorapunda, and Haematobia irritans. This low sequence similarity is comparable to that found among mariner-like elements in all insects. However, conserved amino acid motifs were not discovered nor was a single open reading frame found. These aspects suggest that the An. albimanus,mariner-like element may represent an ancient transposition event but that the element is no longer active, the typical open reading frame having been disrupted through nucleotide insertions and deletions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 04:06:22