Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Application of first-derivative UV spectrophotometry, TLC-densitometry andliquid chromatography for the simultaneous determination of mebeverine hydrochloride and sulpiride
Autore:
El Walily, AFM; El Gindy, A; Bedair, MF;
Indirizzi:
Univ Alexandria, Fac Pharm, Dept Pharmaceut Analyt Chem, Alexandria, EgyptUniv Alexandria Alexandria Egypt rmaceut Analyt Chem, Alexandria, Egypt Suez Canal Univ, Fac Pharm, Dept Pharmaceut Analyt Chem, Ismailia, Egypt Suez Canal Univ Ismailia Egypt Pharmaceut Analyt Chem, Ismailia, Egypt Alexandria Co Pharmaceut & Chem Ind, Alexandria, Egypt Alexandria Co Pharmaceut & Chem Ind Alexandria Egypt Alexandria, Egypt
Titolo Testata:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
fascicolo: 3, volume: 21, anno: 1999,
pagine: 535 - 548
SICI:
0731-7085(199911)21:3<535:AOFUST>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
THIN-LAYER CHROMATOGRAPHY; PERFORMANCE; TABLETS; SPECTROSCOPY; HPLC; PH;
Keywords:
first-derivative spectroscopy; liquid chromatography; mebeverine hydrochloride; pharmaceutical tablets; sulpiride; TLC-densitometry; zero-crossing measurements;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: El Walily, AFM Univ Alexandria, Fac Pharm, Dept Pharmaceut Analyt Chem, POB Elmesalah 21 521,Khartoum Sq, Alexandria, Egypt Univ Alexandria POB Elmesalah 21 521,Khartoum Sq Alexandria Egypt
Citazione:
A.F.M. El Walily et al., "Application of first-derivative UV spectrophotometry, TLC-densitometry andliquid chromatography for the simultaneous determination of mebeverine hydrochloride and sulpiride", J PHARM B, 21(3), 1999, pp. 535-548

Abstract

Three methods are described for the simultaneous determination of mebeverine hydrochloride (MB) and sulpiride (SU) in combined pharmaceutical tablets. The first method depends on first-derivative ultraviolet spectrophotometry, with zero-crossing measurement method. The first derivative amplitudes at 214.2 and 221.6 nm were selected for the assay of MB and SU, respectively. Calibration graphs follow Beer's law in the range of 10-30 and 2-8 mu g ml(-1), and the linearity was satisfactory (r = 0.9999), for MB and SU, respectively. The second method was based on the application of the thin layer chromatographic separation of both drugs followed by the densitometric measurements of their spot areas. After separation on silica gel GF254 plates, using ethanol: diethyl ether: triethylamine (70:30:1 v/v) as the mobile phase, the chromatographic zones corresponding to the spots of MB and SU were scanned at 262 and 240 nm, respectively. The calibration function was established in the ranges of 4-12 mu g for MB and 2-8 mu g for SU. The third method was an internal standard procedure based on high performance liquid chromatographic separation of the two drugs on a reversed-phase, Bondapak CN column. The detection was done at 243 nm using buclizine hydrochloride as internal standard. All chromatographic methods showed good linearity, precision and reproducibility. No spectral or chromatographic interference from the tablet excipients were found. The proposed methods were successfully applied to the assay of commercial tablets and content uniformity test. The procedures were rapid, simple acid suitable for quality control application. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 09:50:11