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Titolo:
Growth of normal oral keratinocytes and squamous cell carcinoma cells in anovel protein-free defined medium
Autore:
Kamata, N; Yokoyama, K; Fujimoto, R; Ueda, N; Hayashi, E; Nakanishi, H; Nagayama, M;
Indirizzi:
Univ Tokushima, Sch Dent, Dept Oral & Maxillofacial Surg 1, Tokushima 7708504, Japan Univ Tokushima Tokushima Japan 7708504 Surg 1, Tokushima 7708504, Japan
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 10, volume: 35, anno: 1999,
pagine: 635 - 641
SICI:
1071-2690(199911/12)35:10<635:GONOKA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERUM-FREE MEDIUM; HUMAN EPIDERMAL-KERATINOCYTES; FACTOR-I; CALCIUM REGULATION; CLONAL GROWTH; IGF-I; DIFFERENTIATION; RECEPTOR; CULTURE; STIMULATION;
Keywords:
protein-free culture; defined medium; normal keratinocytes; squamous cell carcinoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Kamata, N Univ Tokushima, Sch Dent, Dept Oral & Maxillofacial Surg 1, 3-18-15 Kuramoto Cho, Tokushima 7708504, Japan Univ Tokushima 3-18-15 Kuramoto Cho Tokushima Japan 7708504 pan
Citazione:
N. Kamata et al., "Growth of normal oral keratinocytes and squamous cell carcinoma cells in anovel protein-free defined medium", IN VITRO-AN, 35(10), 1999, pp. 635-641

Abstract

A novel protein-free synthetic medium was developed for the culture of normal human oral keratinocytes. This medium, designated PFM-7, supports the serial cultivation of primary or secondary normal oral keratinocytes in protein-free, chemically defined conditions. Normal oral keratinocytes in PFM-7exhibited nearly equal growth in mass culture without noticeable changes in morphology, response to added growth factors, or gene expression of growth factors and their receptors, compared to cells in Keratinocyte-SFM containing epidermal growth factor and bovine pituitary extract. Furthermore, PFM-7 supported the serial subcultivation of human squamous cell carcinoma cells and enabled both normal and malignant oral squamous cells derived from the same patient to grow under the same protein-free defined conditions. These results indicate that PFM-7 can be used for precise investigations of growth mechanisms, cell products, and gene expression associated with carcinogenesis of human epidermal cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/02/20 alle ore 14:39:47