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Titolo:
Phenotypic and functional characteristics of porcine peritoneal mesothelial cells
Autore:
Ohan, J; Gilbert, MA; Brouland, JP; Rougier, JP; Trugnan, G; Wassef, M; Leseche, G; Drouet, L;
Indirizzi:
Hop Beaujon, Serv Chirurg Vasc & Thorac Prof G Leseche, Lab Chirurg Vasc &Thorac, F-92100 Clichy, France Hop Beaujon Clichy France F-92100 g Vasc &Thorac, F-92100 Clichy, France INSERM, U353, Paris, France INSERM Paris FranceINSERM, U353, Paris, France Serv Anat Pathol, Paris, France Serv Anat Pathol Paris FranceServ Anat Pathol, Paris, France INSERM, U64, Paris, France INSERM Paris FranceINSERM, U64, Paris, France IFR, INSERM, U96 07, Paris, France IFR Paris FranceIFR, INSERM, U96 07, Paris, France
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 10, volume: 35, anno: 1999,
pagine: 625 - 634
SICI:
1071-2690(199911/12)35:10<625:PAFCOP>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SODIUM DODECYL-SULFATE; DIAMETER DACRON GRAFTS; ENDOTHELIAL-CELLS; GROWTH-FACTORS; FIBRINOLYTIC PROPERTIES; IN-VITRO; TISSUE; CULTURE; EXPRESSION; PROLIFERATION;
Keywords:
peritoneal mesothelial porcine cells; electron microscopy; intermediate filaments; plasminogen activator; metalloproteinase production;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Ohan, J Hop Beaujon, Serv Chirurg Vasc & Thorac Prof G Leseche, Lab Chirurg Vasc &Thorac, 9eme Etage,100 Blvd Gen Leclerc, F-92100 Clichy, France HopBeaujon 9eme Etage,100 Blvd Gen Leclerc Clichy France F-92100
Citazione:
J. Ohan et al., "Phenotypic and functional characteristics of porcine peritoneal mesothelial cells", IN VITRO-AN, 35(10), 1999, pp. 625-634

Abstract

The objective of this study was to establish a technique to isolate porcine mesothelial cells (PMCs) from omental tissue and to compare them to humanmesothelial cells (HMCs). The PMCs were dispersed by collagenase digestionand isolated on a Ficoll(R) layer. Their morphologic and ultrastructural features were assessed at confluence by light and electronic microscopy, andthey were characterized by immunohistochemistry using specific HMC markers. PMC proliferation was studied in the presence of growth factors platelet-derived growth factor (PDGF), epidermal growth factor (EGF) or transforminggrowth factors beta 1, beta 2, or beta 3 (TGF). Fibrinolytic PMC activity was detected by zymography for tissue plasminogen activator (tPA) and by reverse zymography for plasminogen activator inhibitor-1 (PAI-1). The recalcification time of cell lysates was used to define PMC procoagulant activity,and gelatinase zymography was used to detect metalloproteinase production. At confluence, PMCs formed typical cobblestone monolayers and exhibited structural features characteristic of HMCs. Weibel Palade bodies were never seen. Specific HMC markers (HBME1, ME1, WT1) cross-reacted with PMCs. As HMCs and PMCs coexpressed cytokeratin and vimentin, and also expressed vinculin and alpha-actin. Addition of PDGF or EGF to the culture medium stimulatedPMC proliferation. PMCs constitutively expressed fibrinolytic and procoagulant activity and secreted MMP9 and MMP2. The technique described in this study allows isolation of mesothelial cells from porcine omental tissue. These porcine cells exhibit a mesothelial phenotype and functional properties similar to those of HMCs. Our data warrant an evaluation of mesothelial cells as targets in several therapeutic strategies with porcine models.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 16:19:46