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Titolo:
A sensitive, nonradioactive method for the detection of a low level of apoptosis in rat ovary and human placenta - Technical note
Autore:
Furuta, I; Yamada, H; Sakuragi, N; Fujimoto, S;
Indirizzi:
Hokkaido Univ, Sch Med, Dept Obstet & Gynecol, Kita Ku, Sapporo, Hokkaido 060, Japan Hokkaido Univ Sapporo Hokkaido Japan 060 Ku, Sapporo, Hokkaido 060, Japan
Titolo Testata:
GYNECOLOGIC AND OBSTETRIC INVESTIGATION
fascicolo: 4, volume: 48, anno: 1999,
pagine: 259 - 262
SICI:
0378-7346(1999)48:4<259:ASNMFT>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA FRAGMENTATION;
Keywords:
apoptosis; ovary; placenta; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Furuta, I Hokkaido Univ, Sch Med, Dept Obstet & Gynecol, Kita Ku, N15 W7, Sapporo, Hokkaido 060, Japan Hokkaido Univ N15 W7 Sapporo Hokkaido Japan 060 kaido 060, Japan
Citazione:
I. Furuta et al., "A sensitive, nonradioactive method for the detection of a low level of apoptosis in rat ovary and human placenta - Technical note", GYNECOL OBS, 48(4), 1999, pp. 259-262

Abstract

Apoptosis is often characterized by internucleosomal DNA fragmentation, which is classically visualized in standard agarose gel electrophoresis. However, this technique is not sufficiently sensitive for the detection of a modest apoptotic level in intact tissues, We developed a sensitive, nonradioactive method for the qualitative and quantitative analyses of apoptotic DNAfragmentation is intact tissues. An ultrasensitive chemiluminescent substrate, CDP-Star, was used for the visualization of digoxigenin (DIG)-labeled DNA fragments, and banding patterns were densitometrically quantified. Serially diluted DNA samples from rat ovaries were labeled with DIG. As a result, only 1.56 ng of DNA could be analyzed for the presence of apoptotic DNA cleavage, The sum intensity of these bands increased almost linearly by increasing the amount of labeled DNA, In human placental tissues, clear apoptotic DNA ladders were visualized by this method, and the quantification of apoptotic DNA fragmentation was also possible, This new method provides a nonradioactive, highly sensitive and semiquantitative analysis of apoptotic DNA fragmentation and may be highly useful for the study of a low, physiological level of apoptosis. Copyright (C) 1999 S.Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 08:11:56