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Titolo:
Isolation of microsatellite and RAPD markers flanking the Yr15 gene of wheat using NILs and bulked segregant analysis
Autore:
Chague, V; Fahima, T; Dahan, A; Sun, GL; Korol, AB; Ronin, YI; Grama, A; Roder, MS; Nevo, E;
Indirizzi:
Univ Haifa, Inst Evolut, IL-31905 Haifa, Israel Univ Haifa Haifa Israel IL-31905 fa, Inst Evolut, IL-31905 Haifa, Israel Agr Res Org, Volcani Ctr, IL-50250 Bet Dagan, Israel Agr Res Org Bet Dagan Israel IL-50250 ni Ctr, IL-50250 Bet Dagan, Israel Inst Plant Genet & Crop Plant Res, D-06466 Gatersleben, Germany Inst PlantGenet & Crop Plant Res Gatersleben Germany D-06466 n, Germany Univ Paris Sud, Inst Biotechnol Plantes, F-91405 Orsay, France Univ Paris Sud Orsay France F-91405 chnol Plantes, F-91405 Orsay, France Sichuan Agr Univ, Triticeae Res Inst, Dujiangyan 611830, Sichuan, Peoples R China Sichuan Agr Univ Dujiangyan Sichuan Peoples R China 611830 eoples R China
Titolo Testata:
GENOME
fascicolo: 6, volume: 42, anno: 1999,
pagine: 1050 - 1056
SICI:
0831-2796(199912)42:6<1050:IOMARM>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
RUST RESISTANCE GENE; EMMER TRITICUM-DICOCCOIDES; MOLECULAR MARKERS; STRIPE RUST; WILD EMMER; PUCCINIA-STRIIFORMIS; CHROMOSOMAL LOCATION; HEXAPLOID WHEATS; YELLOW RUST; IDENTIFICATION;
Keywords:
wheat; Triticum dicoccoides; Yr15 stripe rust resistance gene; genetic mapping; microsatellite markers; RAPD markers;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Fahima, T Univ Haifa, Inst Evolut, Mt Carmel, IL-31905 Haifa, Israel Univ Haifa Mt Carmel Haifa Israel IL-31905 -31905 Haifa, Israel
Citazione:
V. Chague et al., "Isolation of microsatellite and RAPD markers flanking the Yr15 gene of wheat using NILs and bulked segregant analysis", GENOME, 42(6), 1999, pp. 1050-1056

Abstract

Microsatellite and random amplified polymorphic DNA (RAPD) primers were used to identify molecular markers linked to the Yr15 gene which confer resistance to stripe rust (Puccina striiformis Westend) in wheat. By using near isogenic lines (NILs) for the Yr15 gene and a F-2 mapping population derived from crosses of these lines and phenotyped for resistance, we identified one microsatellite marker (GWM33) and one RAPD marker (OPA19(800)) linked to Yr15. Then, bulked segregant analysis was used in addition to the NILs toidentify RAPD markers linked to the target gene. Using this approach, two RAPD markers linked to Yr15 were identified, one in coupling (UBC199(700)) and one in repulsion phase (UBC212(1200)). After Mapmaker linkage analysis on the F-2 population, the two closest markers were shown to be linked to Yr15 within a distance of about 12 cM. The recombination rates were recalculated using the maximum likelihood technique to take into account putative escaped individuals from the stripe rust resistance test and obtain unbiaseddistance estimates. As a result of this study, the stripe rust resistance gene Yr15 is surrounded by two flanking PCR markers, UBC199(700) and GWM33,at about 5 cM from each side.

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Documento generato il 21/09/20 alle ore 04:42:02