Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Nitric oxide and peroxynitrite. The ugly, the uglier and the not so good -A personal view of recent controversies
Autore:
Halliwell, B; Zhao, K; Whiteman, M;
Indirizzi:
Natl Univ Singapore, Dept Biochem, Singapore 119260, Singapore Natl Univ Singapore Singapore Singapore 119260 ngapore 119260, Singapore Univ London Kings Coll, Int Antioxidant Res Ctr, London SW3 6LX, England Univ London Kings Coll London England SW3 6LX r, London SW3 6LX, England
Titolo Testata:
FREE RADICAL RESEARCH
fascicolo: 6, volume: 31, anno: 1999,
pagine: 651 - 669
SICI:
1071-5762(1999)31:6<651:NOAPTU>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMYOTROPHIC-LATERAL-SCLEROSIS; HYDROXYL RADICAL PRODUCTION; HUMAN ATHEROSCLEROTIC LESIONS; LOW-MOLECULAR-WEIGHT; SMOOTH-MUSCLE CELLS; TYROSINE NITRATION; HYPOCHLOROUS ACID; HYDROGEN-PEROXIDE; SPINAL-CORD; IN-VIVO;
Keywords:
nitric oxide; peroxynitrite; deamination; 3-nitrotyrosine; xanthine; hypoxanthine; reactive nitrogen species; gastric cancer; lipid peroxidation; superoxide; hydroxyl radical; nitrous acid; nitrite; catechins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
124
Recensione:
Indirizzi per estratti:
Indirizzo: Halliwell, B Natl Univ Singapore, Dept Biochem, 10 Kent Ridge Crescent, Singapore 119260, Singapore Natl Univ Singapore 10 Kent Ridge Crescent Singapore Singapore 119260
Citazione:
B. Halliwell et al., "Nitric oxide and peroxynitrite. The ugly, the uglier and the not so good -A personal view of recent controversies", FREE RAD RE, 31(6), 1999, pp. 651-669

Abstract

Nitric oxide, a gaseous free radical, is poorly reactive with most biomolecules but highly reactive with other free radicals. Its ability to scavengeperoxyl and other damaging radicals may make it an important antioxidant in vivo, particularly in the cardiovascular system, although this ability has been somewhat eclipsed in the literature by a focus on the toxicity of peroxynitrite, generated by reaction of O-2(.-) with NO. (or of NO- with O-2)On balance, experimental and theoretical data support the view that ONOO- can lead to hydroxyl radical (OH.) generation at pH 7.4, but it seems unlikely that OH. contributes much to the cytotoxicity of ONOO-. The cytotoxicity of ONOO- may have been over-emphasized: its formation and rapid reaction with antioxidants may provide a mechanism of using NO . to or even of usingO-2(.) to dispose of dispose of excess O-2(.-) excess NO., in order to maintain the correct balance between these radicals in vivo. Injection or instillation of "bolus" ONOO- into animals has produced tissue injury, however,although more experiments generating ONOO- at steady rates in vivo are required. The presence of S-nitrotyrosine in tissues is still frequently takenas evidence of ONOO- generation in vivo but abundant evidence now exists to support the view that it is a biomarker of several "reactive nitrogen species" Another under-addressed problem is the reliability of assays used to detect and measure 3-nitrotyrosine in tissues and body fluids: immunostaining results vary between laboratories and simple HPLC methods are susceptible to artefacts. Exposure of biological material to low pH (e.g. during acidic hydrolysis to liberate nitrotyrosine from proteins) or to H2O2 might cause artefactual generation of nitrotyrosine from NO2- in the samples. This may be the origin of some of the very large values for tissue nitrotyrosine levels quoted in the literature. Nitrous acid causes not only tyrosine nitration but also DNA base deamination at low pH: these events are relevant tothe human stomach since saliva and many foods are rich in nitrite. Severalplant phenolics inhibit nitration and deamination in vitro, an effect thatcould conceivably contribute to their protective effects against gastric cancer development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 08:14:46