Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Nitric oxide as a modulator of central respiratory rhythm in the isolated brainstem of the bullfrog (Rana catesbeiana)
Autore:
Hedrick, MS; Morales, RD;
Indirizzi:
Calif State Univ Hayward, Dept Sci Biol, Hayward, CA 94542 USA Calif StateUniv Hayward Hayward CA USA 94542 Biol, Hayward, CA 94542 USA
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
fascicolo: 3, volume: 124, anno: 1999,
pagine: 243 - 251
SICI:
1095-6433(199911)124:3<243:NOAAMO>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; MEMBRANE-PROPERTIES; NEURAL ACTIVITY; CONSCIOUS RAT; GENERATION; MAMMALS; STEM; FROG; MECHANISMS; RESPONSES;
Keywords:
nitric oxide; breathing; central pattern generator; amphibian; L-arginine; 7-nitro indazole; sodium nitroprusside; nNOS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Hedrick, MS Calif State Univ Hayward, Dept Sci Biol, Hayward, CA 94542 USACalif State Univ Hayward Hayward CA USA 94542 d, CA 94542 USA
Citazione:
M.S. Hedrick e R.D. Morales, "Nitric oxide as a modulator of central respiratory rhythm in the isolated brainstem of the bullfrog (Rana catesbeiana)", COMP BIOC A, 124(3), 1999, pp. 243-251

Abstract

Nitric oxide (NO) is a unique interneuronal neurotransmitter and/or neuromodulator that is involved in a variety of physiological functions within the central nervous system (CNS). In neural tissue, NO is generated from an oxygen-dependent, constitutive NO synthase (NOS) by glutamatergic stimulation of A7-methyl-D-aspartate (NMDA) receptors. Recent studies indicate that NO has excitatory effects on breathing within the CNS and mediates a centralcomponent of the hypoxic ventilatory reflex in mammals. Because NMDA receptors are important in central respiratory rhythmogenesis, we hypothesized that NO would have significant effects on the central pattern generator (CPG) for breathing in the brainstem. To test this hypothesis, the effects of NO on respiratory-related neural activity were investigated using an in vitro brainstem preparation from North American bullfrogs (liana catesbeiana). Extracellular recordings of respiratory-related burst activity were made from cranial nerves V, X and XII before and during superfusion of the brainstem with NO-generating compounds, or inhibitors of NO synthesis. Addition ofthe NO donor, sodium nitroprusside (SNP; 0.1-1.0 mM), or the amino acid precursor for NO synthesis, L-arginine (L-Arg; 0.01-1.0 mM), caused significant increases in respiratory-related burst frequency. Inhibition of NOS withN-omega-nitro-L-arginine (L-NA; 5-10 mM), a-non-selective NOS inhibitor, caused a significant reduction in burst frequency or reversibly abolished neural activity. Brainstem perfusion with the specific neuronal NOS (nNOS) inhibitor, 7-nitro indazole (7-NI), produced significant, dose-dependent reversible reductions in burst frequency at concentrations of 0.1, 0.5 and 1.0 mM. These results suggest that production of NO, probably via nNOS, provides an excitatory input to the respiratory CPG in the amphibian brainstem. Our results suggest that NO may be a necessary inter- or intracellular messenger for neurotransmission and/or neuromodulation of central respiratory drive to motor effecters in the bullfrog. (C) 1999 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 06:34:53