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Titolo:
Transglutaminase and coeliac disease: endomysial reactivity and small bowel expression
Autore:
Brusco, G; Muzi, P; Ciccocioppo, R; Biagi, F; Cifone, MG; Corazza, GR;
Indirizzi:
Univ Pavia, Policlin San Matteo, IRCCS, Gastroenterol Unit, I-27100 Pavia,Italy Univ Pavia Pavia Italy I-27100 , Gastroenterol Unit, I-27100 Pavia,Italy Univ Aquila, Dept Expt Med, I-67100 Laquila, Italy Univ Aquila Laquila Italy I-67100 Dept Expt Med, I-67100 Laquila, Italy
Titolo Testata:
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
fascicolo: 3, volume: 118, anno: 1999,
pagine: 371 - 375
SICI:
0009-9104(199912)118:3<371:TACDER>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELIAC-DISEASE; TISSUE TRANSGLUTAMINASE; GLIADIN; AUTOANTIBODIES; FOOD; AUTOANTIGEN; ANTIBODIES; SERUM; CELLS; RAT;
Keywords:
coeliac disease; tissue transglutaminase; endomysial antibodies;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Corazza, GR Univ Pavia, Policlin San Matteo, IRCCS, Gastroenterol Unit, P Golgi 19, I-27100 Pavia, Italy Univ Pavia P Golgi 19 Pavia Italy I-27100 -27100 Pavia, Italy
Citazione:
G. Brusco et al., "Transglutaminase and coeliac disease: endomysial reactivity and small bowel expression", CLIN EXP IM, 118(3), 1999, pp. 371-375

Abstract

This study was aimed at verifying whether tissue transglutaminase (tTG) isthe sole autoantigen eliciting anti-endomysial antibodies in coeliac disease (CoD) and investigating tTG expression in normal and coeliac mucosa. Twelve anti-endomysial-positive coeliac sera and 12 anti-endomysial-negative control sera (10 mu l, diluted 1:5-1:400 in PBS pH 7.3) were preincubated with 10, 20 or 50 mu g guinea pig liver tTG at 4 degrees C overnight. Monkey oesophagus tissue slides were then tested with tTG-preincubated and non-preincubated sera to search for IgA anti-endomysial reactivity by indirect immunofluorescence. Moreover, six sections of monkey oesophagus were incubatedwith an anti-tTG mouse MoAb, six sections with an anti-cytokeratin mouse MoAb and six sections with only 3% bovine serum albumin. Finally, endoscopicduodenal biopsy sections obtained from 12 patients affected by untreated CoD, six patients affected by treated CoD and 10 biopsied controls were immunohistochemically stained with a peroxidase-conjugated anti-tTG MoAb. Our results show that (i) preincubation with tTG abolished endomysial immunofluorescence in most, but not in all, coeliac sera; (ii) the incubation of anti-tTG MoAb with sections of monkey oesophagus resulted in an immunofluorescence staining pattern similar but not identical to that of anti-endomysial-positive coeliac sera; (iii) although tTG expression was present at muscularis mucosae and pericryptal fibroblast in both normal and coeliac mucosa, itwas slightly more marked and evident in the latter. Although our absorption experiment was performed with guinea pig liver tTG, we confirm that tTG is the predominant antigen of endomysial antibodies, but we speculate that, at least in some patients, it is not the only one.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 11:34:55