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Titolo:
The alpha 1-6-fucosyltransferase gene and its biological significance
Autore:
Miyoshi, E; Noda, K; Yamaguchi, Y; Inoue, S; Ikeda, Y; Wang, WG; Ko, JH; Uozumi, N; Li, W; Taniguchi, N;
Indirizzi:
Osaka Univ, Sch Med, Dept Biochem, Suita, Osaka 5650871, Japan Osaka UnivSuita Osaka Japan 5650871 Biochem, Suita, Osaka 5650871, Japan
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
fascicolo: 1, volume: 1473, anno: 1999,
pagine: 9 - 20
SICI:
0304-4165(199912)1473:1<9:TA1GAI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
N-ACETYLGLUCOSAMINYLTRANSFERASE-V; ALPHA-1-6 FUCOSYL-TRANSFERASE; SIALYL-LEWIS-X; GROUP ALPHA(1,2)FUCOSYLTRANSFERASE GENE; HUMAN ALPHA(1,3)FUCOSYLTRANSFERASE GENE; LECTIN AFFINITY ELECTROPHORESIS; CYSTIC-FIBROSIS FIBROBLASTS; LINKED SUGAR CHAINS; GDP-L-FUCOSE; HEPATOCELLULAR-CARCINOMA;
Keywords:
alpha 1-6-fucosyltransferase; alpha-fetoprotein; hepatoma; platelet;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
93
Recensione:
Indirizzi per estratti:
Indirizzo: Taniguchi, N Osaka Univ, Sch Med, Dept Biochem, Room B-1,2-2 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ Room B-1,2-2 Yamadaoka Suita Osaka Japan 5650871 n
Citazione:
E. Miyoshi et al., "The alpha 1-6-fucosyltransferase gene and its biological significance", BBA-GEN SUB, 1473(1), 1999, pp. 9-20

Abstract

GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha 1-6-fucosyltransferase (alpha 1-6FucT) catalyzes the transfer of fucose from GDP-Fuc to N-linked type complex glycoproteins. This enzyme was purified from a human fibroblast cellline, porcine brain, a human gastric cancer cell line and human blood platelets. cDNA cloning of porcine and human alpha 1-6FucT was performed from aporcine brain and gastric cancer cell cDNA libraries, respectively. Their homology is 92.2% at the nucleotide level and 95.7% at the amino acid level. No putative N-glycosylation sites were found in the predicted amino acid sequence. No homology to other fucosyltransferases such as alpha 1-2FucT, alpha 1-3FucT and alpha 1-4FucT was found except for a region consisting of nine amino acids. The alpha 1-GFucT gene is located at chromosome 14q24.3, which is also a different location from other fucosyltransferases reported to date. The alpha 1-GFucT gene is the oldest gene family in the phylogenictrees among the nine cloned fucosyltransferase genes. alpha 1-GFucT is widely expressed in various rat tissues and the expression of alpha 1-GFucT inthe liver is enhanced during hepatocarcinogenesis of LEC rats which develop hereditary hepatitis and hepatomas. In cases of human liver diseases, alpha 1-6FucT is expressed in both hepatoma tissues and their surrounding tissues with chronic liver disease, but not in the case of normal liver. Serum alpha 1-6-fucosylated alpha-fetoprotein (AFP) has been employed for an early diagnosis of patients with hepatoma. The mechanisms by which alpha 1-6 fucosylation of AFP occurs in the hepatoma is not due to the up-regulation ofalpha 1-6FucT alone. Interestingly, when the alpha 1-GFucT gene is transfected into Hep3B, a human hepatoma cell line, tumor formation in the liver of nude mice after splenic injection is dramatically suppressed. In this review, we focus on alpha 1-6FucT and summarize its properties, gene expression and biological significance. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 23:13:46