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Titolo:
Asymmetric distribution of muscarinic acetylcholine receptors in Madin-Darby canine kidney cells
Autore:
Nadler, LS; Kumar, G; Hinds, TR; Migeon, JC; Nathanson, NM;
Indirizzi:
Univ Washington, Sch Med, Dept Pharmacol, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 ept Pharmacol, Seattle, WA 98195 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 6, volume: 277, anno: 1999,
pagine: C1220 - C1228
SICI:
0363-6143(199912)277:6<C1220:ADOMAR>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
MDCK-CELLS; CHOLINERGIC RECEPTORS; FUNCTIONAL EXPRESSION; XENOPUS OOCYTES; ACINAR-CELLS; IDENTIFICATION; SEQUENCE; MEMBRANE; GENE; LOCALIZATION;
Keywords:
muscarinic receptor; cell polarity; adenosine triphosphate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Nathanson, NM Univ Washington, Sch Med, Dept Pharmacol, Box 357750, Seattle, WA 98195 USA Univ Washington Box 357750 Seattle WA USA 98195 WA 98195 USA
Citazione:
L.S. Nadler et al., "Asymmetric distribution of muscarinic acetylcholine receptors in Madin-Darby canine kidney cells", AM J P-CELL, 277(6), 1999, pp. C1220-C1228

Abstract

We have characterized the muscarinic ACh receptors (mAChRs) expressed in Madin-Darby canine kidney (MDCK) strain II epithelial cells. Binding studieswith the membrane-impermeable antagonist N-[H-3]-methylscopolamine demonstrated that mAChRs are similar to 2.5 times more abundant on the basolateralthan on the apical surface. Apical, but not basolateral, mAChRs inhibited forskolin-stimulated adenylyl cyclase activity in response to the agonist carbachol. Neither apical nor basolateral mAChRs exhibited detectable carbachol-stimulated phospholipase C activity. Carbachol application to the apical or the basolateral membrane resulted in a threefold increase in intracellular Ca2+ concentration, which was completely inhibited by pertussis toxin on the apical side and partially inhibited on the basolateral side. RT-PCR analysis showed that MDCK cells express the M-4 and M-5 receptor mRNAs. These data suggest that M-4 receptors reside an the apical and basolateral membranes of polarized MDCK strain IE cells and that the M-5 receptor may reside in the basolateral membrane of a subset of cells.

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Documento generato il 27/11/20 alle ore 06:59:38