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Titolo:
Proteoglycan composition and biosynthesis in superficial and deep digital flexor tendons of standardbred horses
Autore:
Micklethwaite, L; Ghosh, P; Little, CB; Hodgson, DR; Rose, RJ;
Indirizzi:
Univ Sydney, Royal N Shore Hosp, Inst Bone & Joint Dis, St Leonards, NSW 2065, Australia Univ Sydney St Leonards NSW Australia 2065 Leonards, NSW 2065, Australia Univ Sydney, Royal N Shore Hosp, Dept Surg, St Leonards, NSW 2065, Australia Univ Sydney St Leonards NSW Australia 2065 Leonards, NSW 2065, Australia Univ Sydney, Rural Vet Ctr, Equine Performance Lab, Camden, NSW, AustraliaUniv Sydney Camden NSW Australia Performance Lab, Camden, NSW, Australia
Titolo Testata:
VETERINARY AND COMPARATIVE ORTHOPAEDICS AND TRAUMATOLOGY
fascicolo: 4, volume: 12, anno: 1999,
pagine: 166 - 172
SICI:
0932-0814(199912)12:4<166:PCABIS>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCOSAMINOGLYCAN CONTENT; ARTICULAR-CARTILAGE; MECHANICAL FORCES; BOVINE TENDON; COLLAGEN; MORPHOLOGY; LIGAMENTS; PROTEINS; REGIONS; ASSAY;
Keywords:
equine; proteoglycans; biosynthesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Ghosh, P Univ Sydney, Royal N Shore Hosp, Inst Bone & Joint Dis, St Leonards, NSW 2065, Australia Univ Sydney St Leonards NSW Australia 2065 , NSW 2065, Australia
Citazione:
L. Micklethwaite et al., "Proteoglycan composition and biosynthesis in superficial and deep digital flexor tendons of standardbred horses", VET COMP OR, 12(4), 1999, pp. 166-172

Abstract

Proteoglycan (PG) levels and their respective biosynthesis were determinedin explant cultures taken from the mid-metacarpal (MMC) and metacarpophalangeal (MCP) regions of normal equine Superficial and deep digital flexor tendons. Five standard-bred horses (2-5 years old) were used and explants dissected from the MMC and MCP regions of the superficial and deep digital flexor tendons. The explants (3 mm(2)) were cultured in Ham's F12 media supplemented with 1% FBS. After 24 h, (Na2SSO4)-S-35 was added and the culture continued for a further 48 h. The media was removed and the explants were lyophilized and weighed. The explants and media were then subject to papain digestion and the DNA and sulphated glycosaminoglycans (S-GAG) concentrationsand incorporation of [S-35] sulphate into PGs determined by established methods. The MMC regions of the superficial and deep digital flexor tendons contained less PGs than the MCP regions and these were predominantly small. The MCP regions, not only contained higher concentrations of PGs, but these were of larger hydrodynamic size. The incorporation of [S-35] sulphate into PGs was also higher in explants from the MCP region. These data indicate that in those regions of equine flexor tendon, subjected to both, compression andtension, PG content and biosynthesis is higher than in regions which only transmit tensional loads. These findings are consistent with the adaptive role of tenocytes to the nature and level of the mechanical stresses imposedupon them.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 09:30:59