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Titolo:
Purification and some properties of a tetrodotoxin binding protein from the blood plasma of kusafugu, Takifugu niphobles
Autore:
Matsui, T; Yamamori, K; Furukawa, K; Kono, M;
Indirizzi:
Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 Life Sci, Bunkyo Ku, Tokyo 1138657, Japan Kitasato Univ, Sch Fisheries Sci, Sanriku, Iwate 0220101, Japan Kitasato Univ Sanriku Iwate Japan 0220101 , Sanriku, Iwate 0220101, Japan Univ Tokyo, Fac Agr, Fisheries Res Lab, Shizuoka 4310201, Japan Univ Tokyo Shizuoka Japan 4310201 eries Res Lab, Shizuoka 4310201, Japan
Titolo Testata:
TOXICON
fascicolo: 3, volume: 38, anno: 2000,
pagine: 463 - 468
SICI:
0041-0101(200003)38:3<463:PASPOA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANHYDROTETRODOTOXIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
7
Recensione:
Indirizzi per estratti:
Indirizzo: Matsui, T Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 Bunkyo Ku, Tokyo 1138657, Japan
Citazione:
T. Matsui et al., "Purification and some properties of a tetrodotoxin binding protein from the blood plasma of kusafugu, Takifugu niphobles", TOXICON, 38(3), 2000, pp. 463-468

Abstract

A tetrodotoxin binding protein has been purified from the plasma of the puffer fish kusafugu, Takifugu niphobles, through DEAF-cellulose treatment, ammonium sulfate fractionation, Sephadex gelfiltrations and Sephacryl S-200 and Cellulofine A-500 column chromatography, Final purification by HPLC on a TSK G-3000 SL column yielded a protein which showed only a. single protein peak, The molecular weight of the protein was estimated to be 116,000 and91,000 by SDS-PAGE and mass spectrometry, respectively. A blast search on the amino-terminal amino acid sequence of the purified protein revealed that the protein had no homology to any other protein on data base. (C) 1999 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 10:12:23