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Titolo:
Effect of morphine on cholecystokinin and mu-opioid receptor-like immunoreactivities in rat spinal dorsal horn neurons after peripheral axotomy and inflammation
Autore:
Zhang, X; Lucas, GD; Elde, R; Wiesenfeld-Hallin, Z; Hokfelt, T;
Indirizzi:
Karolinska Inst, Dept Neurosci, Stockholm, Sweden Karolinska Inst Stockholm Sweden Inst, Dept Neurosci, Stockholm, Sweden Huddinge Univ Hosp, Dept Med Lab Sci & Technol, Div Clin Neurophysiol, Stockholm, Sweden Huddinge Univ Hosp Stockholm Sweden lin Neurophysiol, Stockholm, Sweden Fourth Mil Med Univ, Inst Neurosci, Xian 710032, Peoples R China Fourth Mil Med Univ Xian Peoples R China 710032 710032, Peoples R China Univ Minnesota, Dept Cell Biol & Neuroanat, Minneapolis, MN 55455 USA UnivMinnesota Minneapolis MN USA 55455 roanat, Minneapolis, MN 55455 USA
Titolo Testata:
NEUROSCIENCE
fascicolo: 1, volume: 95, anno: 2000,
pagine: 197 - 207
SICI:
0306-4522(2000)95:1<197:EOMOCA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROOT GANGLION NEURONS; GENE-RELATED PEPTIDE; VASOACTIVE INTESTINAL POLYPEPTIDE; CARRAGEENAN-INDUCED INFLAMMATION; PRIMARY SENSORY NEURONS; IN-SITU HYBRIDIZATION; DOWN-REGULATION; MESSENGER-RNAS; BINDING-SITES; FUNCTIONAL IMPLICATIONS;
Keywords:
analgesia; co-existence; nerve injury; pain; spinal cord;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
78
Recensione:
Indirizzi per estratti:
Indirizzo: Hokfelt, T Karolinska Inst, Dept Neurosci, Stockholm, Sweden Karolinska Inst Stockholm Sweden Neurosci, Stockholm, Sweden
Citazione:
X. Zhang et al., "Effect of morphine on cholecystokinin and mu-opioid receptor-like immunoreactivities in rat spinal dorsal horn neurons after peripheral axotomy and inflammation", NEUROSCIENC, 95(1), 2000, pp. 197-207

Abstract

In order to further investigate the interaction between the octapeptide cholecystokinin and opioid analgesia in the spinal cord we used double-colourimmunofluorescence to examine the anatomical distribution of cholecystokinin and mu-opioid receptors in the dorsal horn, as well as the effect of morphine on cholecystokinin- and mu-opioid receptor-like immunoreactivities following peripheral nerve injury and inflammation. mu-opioid receptor-like immunoreactivity was present in 65.6% of cholecystokinin-positive neurons inlaminae I and II of rat spinal cord. Conversely, 40.4% of mu-opioid receptor-positive neurons contained cholecystokinin-like immunoreactivity. Systemic application of morphine (1, 3 or 10 mg/kg; i.v.) after sciatic nerve section significantly, but reversibly, decreased mu-Opioid receptor-like immunoreactivity in the medial half of lamina II in segment L5 of the ipsilateral dorsal horn, and cholecystokinin-like immunoreactivity was also markedly reduced in the same region. These effects were dose- and time-dependent andcould be prevented by naloxone preadministration. In contrast, no significant change in the pattern of distribution or intensity of mu-opioid receptor- and cholecystokinin-like immunoreactivities was observed in intact rats or during peripheral inflammation. These results provide a cellular basis for the interaction of mu-opioid receptors and cholecystokinin at the spinal level by showing a high degree ofco-existence of these two molecules in local interneurons, and also show that morphine can induce rapid and short lasting effects on mu-opioid receptors after peripheral nerve injury. The results contribute to our understanding of how endogenous cholecystokinin reduces the analgesic effect of morphine. (C) 1999 IBRO. Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 09:58:58