Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Novel subdivisions of the rat accessory olfactory bulb revealed by the combined method with lectin histochemistry, electrophysiological and optical recordings
Autore:
Sugai, T; Sugitani, M; Onoda, N;
Indirizzi:
Kanazawa Med Univ, Dept Physiol, Uchinada, Ishikawa 9200293, Japan Kanazawa Med Univ Uchinada Ishikawa Japan 9200293 Ishikawa 9200293, Japan
Titolo Testata:
NEUROSCIENCE
fascicolo: 1, volume: 95, anno: 2000,
pagine: 23 - 32
SICI:
0306-4522(2000)95:1<23:NSOTRA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PUTATIVE PHEROMONE RECEPTORS; VOMERONASAL NERVE-FIBERS; G-PROTEINS; MULTIGENE FAMILY; DIFFERENTIAL LOCALIZATION; CHEMOSENSORY SYSTEMS; PIRIFORM CORTEX; 2 LECTINS; OPOSSUM; IDENTIFICATION;
Keywords:
in vitro; optical imaging; oscillation; Ricinus communis agglutinin (RCA(120)); voltage-sensitive dye;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Sugai, T Kanazawa Med Univ, Dept Physiol, Uchinada, Ishikawa 9200293, Japan Kanazawa Med Univ Uchinada Ishikawa Japan 9200293 9200293, Japan
Citazione:
T. Sugai et al., "Novel subdivisions of the rat accessory olfactory bulb revealed by the combined method with lectin histochemistry, electrophysiological and optical recordings", NEUROSCIENC, 95(1), 2000, pp. 23-32

Abstract

Wistaria floribunda agglutinin and peanut agglutinin were found to bind histochemically to the anterior and posterior regions, respectively, of the vomeronasal nerve and glomerular layers in the rat accessory olfactory bulb. Furthermore, Ricinus communis agglutinin showed strong binding to the anterior region of the vomeronasal nerve and glomerular layers, whereas it bound weakly and/or moderately to the rostral two-thirds of the posterior glomerular layer but not at all to the caudal one-third. This suggests that the posterior region is further divided into two subregions. An electrophysiological mapping study in sagittal slice preparations demonstrated that stimulation given within the anterior vomeronasal nerve layer elicited field potentials within the anterior region of the external plexiform layer, whereas shocks to the rostral two-thirds and the caudal one-third of the posterior vomeronasal nerve layer provoked field responses within the rostral two-thirds and within the caudal one-third of the posterior external plexiform layer, respectively, indicating that the posterior external plexiform layer isalso divided into two subregions. Real-time optical imaging showed similarresults as above, except that neural activity also spread into mitral celllayers. Furthermore, the most anterior and posterior ends of the neural activity evoked in the rostral two-thirds of the posterior region immediatelyadjoined the posterior border of that evoked in the anterior region and the anterior border of that evoked in the caudal one-third of the posterior region, respectively. Moreover, the granule cell layer was also found to have similar boundaries. Thus, optical imaging studies demonstrated individualprecise boundaries of these subdivisions, which were positioned right beneath those defined by Ricinus communis agglutinin histochemistry. The presence of functional segregation in each layer leads us to conclude that there are at least three different input-output pathways in the rat vomeronasal system. (C) 1999 IBRO. Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 09:03:47