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Titolo:
Electrophysiological characterization of rat and mouse olfactory receptor neurons from an intact epithelial preparation
Autore:
Ma, MH; Chen, WR; Shepherd, GM;
Indirizzi:
Yale Univ, Sch Med, Neurobiol Sect, New Haven, CT 06510 USA Yale Univ NewHaven CT USA 06510 Neurobiol Sect, New Haven, CT 06510 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE METHODS
fascicolo: 1-2, volume: 92, anno: 1999,
pagine: 31 - 40
SICI:
0165-0270(19991015)92:1-2<31:ECORAM>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE-GATED CHANNELS; ODORANT RECEPTOR; 2ND-MESSENGER SYSTEM; ACTION-POTENTIALS; TIGER SALAMANDER; SUPPORTING CELLS; CARBON-MONOXIDE; K+ CHANNELS; CURRENTS; ACTIVATION;
Keywords:
olfactory epithelium; olfactory receptor neuron; dendritic knob; odor response; membrane properties; rat; mouse; patch clamp;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Ma, MH Yale Univ, Sch Med, Neurobiol Sect, 333 Cedar St, New Haven, CT 06510 USA Yale Univ 333 Cedar St New Haven CT USA 06510 w Haven, CT 06510 USA
Citazione:
M.H. Ma et al., "Electrophysiological characterization of rat and mouse olfactory receptor neurons from an intact epithelial preparation", J NEUROSC M, 92(1-2), 1999, pp. 31-40

Abstract

To understand the coding mechanisms underlying olfactory discrimination, it is necessary to characterize odor response properties of olfactory receptor neurons (ORNs). In contrast with rapid progress in molecular biology, there is little physiological data from ORNs in rodent. To facilitate acquisition of such data, we have developed an intact olfactory epithelial preparation from both rat and mouse. We have carried out initial studies of this preparation by monitoring odor responses by patch-clamping directly on the ORN dendritic knobs, a subcellular site very close to the locus of olfactorysignal transduction. Our results show that rat and mouse ORNs have similarintrinsic membrane properties. Most cells fired spontaneously at a low frequency (f) and about one half fired repetitively in response to current (I)injection with a linear f/I relation. About one third of rat and mouse ORNs responded to a mixture of four odors in a dose-dependent manner and about60% of them responded to IBMX, a potent inhibitor of phosphodiesterase. The results suggest that this intact preparation offers the advantage of approximating in vivo physiological conditions, while furnishing an opportunityto map single neuron responses in the epithelium in a spatially-defined manner, using electrophysiological or cell imaging methods. (C) 1999 ElsevierScience B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 12:27:02