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Titolo:
Axon regeneration in organotypic slice cultures from the mammalian auditory system is topographic and functional
Autore:
Lohmann, C; Ehrlich, I; Friauf, E;
Indirizzi:
Univ Frankfurt Klinikum, Zentrum Physiol, D-60590 Frankfurt, Germany Univ Frankfurt Klinikum Frankfurt Germany D-60590 590 Frankfurt, Germany
Titolo Testata:
JOURNAL OF NEUROBIOLOGY
fascicolo: 4, volume: 41, anno: 1999,
pagine: 596 - 611
SICI:
0022-3034(199912)41:4<596:ARIOSC>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPERIOR OLIVARY COMPLEX; GANGLION-CELL AXONS; ELECTRICAL MEMBRANE-PROPERTIES; BRAIN-STEM; GLYCINERGIC TRANSMISSION; TRAPEZOID BODY; SPINAL-CORD; IN-VITRO; MEDIAL NUCLEUS; NEURITE GROWTH;
Keywords:
superior olivary complex; lateral superior olive; medial nucleus of trapezoid body; glycine; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Friauf, E Washington Univ, Sch Med, Dept Anat & Neurobiol, 660 S Euclid Ave, St Louis, MO 63110 USA Washington Univ 660 S Euclid Ave St Louis MO USA 63110 63110 USA
Citazione:
C. Lohmann et al., "Axon regeneration in organotypic slice cultures from the mammalian auditory system is topographic and functional", J NEUROBIOL, 41(4), 1999, pp. 596-611

Abstract

In vitro models have frequently been employed to investigate the specificity of the formation of axonal projections during both development and regeneration. Such studies demonstrated pathway, target, and laminar specificity, yet they did not tackle the problem of topography, Here, we addressed theissue of regeneration of spatial specificity at the topographic level by lesioning a precisely organized projection from the auditory system of neonatal rats in organotypic slice culture and by analyzing regeneration capacity, Lesioning had no effect on the survival of axotomized neurons or the structure of the auditory nuclei. Anterograde and retrograde biocytin tracing demonstrated that the projection regenerated topographically at the supracellular level. Whole-cell patch-clamp recordings revealed that the regenerated projection was functional. Topographic regeneration was not impaired by blocking spike activity with tetrodotoxin or glycinergic transmission with strychnine, However, if lesioning was pel formed after the slices had been incubated for 1 week, regeneration capacity was lost despite good survival of neurons. The loss of the regeneration capacity in vitro occurs at a developmental stage that corresponds to the age when the capacity for axonal reorganization is lost in vivo. We conclude that the developmental processes occurring in vivo and irt vitro are comparable in this system, which is whywe think that essential aspects of the loss of regeneration capacity may be addressed with our culture model in the future. (C) 1999 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 09:24:00