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Titolo:
Identification of the hemidesmosomal 500 kDa protein (HD1) as plectin
Autore:
Okumura, M; Uematsu, J; Hirako, Y; Nishizawa, Y; Shimizu, H; Kido, N; Owaribe, K;
Indirizzi:
Nagoya Univ, Unit Biosyst, Grad Sch Human Informat, Nagoya, Aichi 4648601,Japan Nagoya Univ Nagoya Aichi Japan 4648601 ormat, Nagoya, Aichi 4648601,Japan Suzuka Univ Med Sci, Fac Hlth Sci, Suzuka 5100293, Japan Suzuka Univ Med Sci Suzuka Japan 5100293 Hlth Sci, Suzuka 5100293, Japan Hokkaido Univ, Sch Med, Dept Dermatol, Sapporo, Hokkaido 0608638, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608638 oro, Hokkaido 0608638, Japan Natl Inst Basic Biol, Okazaki, Aichi 4448585, Japan Natl Inst Basic Biol Okazaki Aichi Japan 4448585 ki, Aichi 4448585, Japan
Titolo Testata:
JOURNAL OF BIOCHEMISTRY
fascicolo: 6, volume: 126, anno: 1999,
pagine: 1144 - 1150
SICI:
0021-924X(199912)126:6<1144:IOTH5K>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
BULLOUS PEMPHIGOID ANTIGEN; EPIDERMOLYSIS-BULLOSA; MUSCULAR-DYSTROPHY; P34(CDC2) KINASE; EPITHELIAL-CELLS; IMMUNOLOCALIZATION; PHOSPHORYLATION; ORGANIZATION; COMPONENT; ALPHA-6-BETA-4;
Keywords:
cell adhesion; hemidesmosome; HD1; mAb-121; plectin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Okumura, M Nagoya Univ, Unit Biosyst, Grad Sch Human Informat, Nagoya, Aichi 4648601,Japan Nagoya Univ Nagoya Aichi Japan 4648601 ya, Aichi 4648601,Japan
Citazione:
M. Okumura et al., "Identification of the hemidesmosomal 500 kDa protein (HD1) as plectin", J BIOCHEM, 126(6), 1999, pp. 1144-1150

Abstract

HD1 is a 500 kDa hemidesmosomal plaque protein recognized by monoclonal antibody mAb-121, Recent research on inherited skin disease has suggested that it might be identical to plectin or an isoform, To east light on this question, we have prepared several monoclonal antibodies that recognize a 500 kDa protein in the hemidesmosome fraction. Unexpectedly, some staining pattern heterogeneity was observed on immunofluorescence microscopy. Attention was focused on two monoclonal antibodies which gave different localization in bovine skin and retinal pigment epithelial cells. Determination of the amino-terminal sequence of an antigenic 100 kDa polypeptide fragment derivedfrom the 500 kDa component of an insoluble fraction of bovine hepatocytes revealed it was identical to that of plectin. Using the two antibodies, we screened a cDNA library derived from BMGE + H, a bovine mammary gland epithelial cell line, The isolated cDNA clones corresponded to the rod domain ofbovine plectin, with two separate epitope regions for each of the antibodies. From these results we conclude that the hemidesmosomal 500 kDa component HD1 is identical to plectin. As judged on rough estimation of molar ratios on this basis, hemidesmosomes are composed of plectin, BP230, the integrin beta 4 subunit, and alpha 6 in a I:1:1:1 ratio.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 15:56:05