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Titolo:
Involvement of histone phosphorylation in thymocyte apoptosis by protein phosphatase inhibitors
Autore:
Lee, E; Nakatsuma, A; Hiraoka, R; Ishikawa, E; Enomoto, R; Yamauchi, A;
Indirizzi:
Kobe Gakuin Univ, Fac Pharmaceut Sci, Dept Pharmacol, Nishi Ku, Kobe, Hyogo 6512180, Japan Kobe Gakuin Univ Kobe Hyogo Japan 6512180 Ku, Kobe, Hyogo 6512180, Japan Univ Tokushima, Grad Sch Pharmaceut Sci, Dept Pharmaceut Informat Sci, Tokushima 7708505, Japan Univ Tokushima Tokushima Japan 7708505 mat Sci, Tokushima 7708505, Japan
Titolo Testata:
IUBMB LIFE
fascicolo: 1, volume: 48, anno: 1999,
pagine: 79 - 83
SICI:
1521-6543(199907)48:1<79:IOHPIT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYSINE-RICH HISTONE; S-3 CELL-CYCLE; ICE/CED-3 PROTEASE; RAT THYMOCYTES; CORE HISTONES; OKADAIC ACID; CALYCULIN-A; ICE-LIKE; CHROMATIN; HYPERACETYLATION;
Keywords:
apoptosis; chromatin; DNA fragmentation; histone phosphorylation; protein phosphatase inhibitors; thymocyte;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, E Kobe Gakuin Univ, Fac Pharmaceut Sci, Dept Pharmacol, Nishi Ku, Kobe, Hyogo 6512180, Japan Kobe Gakuin Univ Kobe Hyogo Japan 6512180 be, Hyogo 6512180, Japan
Citazione:
E. Lee et al., "Involvement of histone phosphorylation in thymocyte apoptosis by protein phosphatase inhibitors", IUBMB LIFE, 48(1), 1999, pp. 79-83

Abstract

Incubation of rat thymocytes with the inhibitors of protein phosphatase such as calyculin A and okadaic acid resulted in an increase in DNA fragmentation. These effects were dependent on the concentration of the inhibitors and the incubation time. Analyses of the fragmented DNA revealed the production of similar to 50 kbp of DNA and a 180 bp DNA ladder, In addition, a laser scanning-microscopic analysis showed that these compounds caused nuclearcondensation. Thus, these results demonstrated that protein phosphatase inhibitors induced thymocyte apoptosis, The inhibitors of protein phosphataseincreased the phosphorylation of proteins of similar to 15 kDa, The phosphorylation of proteins preceded the DNA fragmentation induced by these inhibitors. Judging from acetic acid-urea-Triton X-100 gel electrophoresis, the phosphorylated proteins were histone H1 and H2A/H3, Therefore, these results suggest that phosphorylation of histones triggers the DNA fragmentation of thymocytes undergoing apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 05:45:53