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Titolo:
Effects of ceramide, the Fas signal intermediate, on apoptosis and phospholipase D activity in mouse ovarian granulosa cells in vitro
Autore:
Kim, JH; Yoon, YD; Shin, I; Han, JS;
Indirizzi:
Hanyang Univ, Inst Biomed Sci, Coll Med, Seoul 133791, South Korea HanyangUniv Seoul South Korea 133791 oll Med, Seoul 133791, South Korea Hanyang Univ, Dept Biochem, Coll Med, Seoul 133791, South Korea Hanyang Univ Seoul South Korea 133791 oll Med, Seoul 133791, South Korea Hanyang Univ, Dept Biol, Coll Nat Sci, Seoul 133791, South Korea Hanyang Univ Seoul South Korea 133791 Nat Sci, Seoul 133791, South Korea
Titolo Testata:
IUBMB LIFE
fascicolo: 4, volume: 48, anno: 1999,
pagine: 445 - 452
SICI:
1521-6543(199910)48:4<445:EOCTFS>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; SPHINGOMYELIN PATHWAY; FOLLICULAR ATRESIA; DEATH; ACTIVATION; INVOLVEMENT; SYSTEM; IDENTIFICATION; PHOSPHATIDATE; FIBROBLASTS;
Keywords:
apoptosis; ceramide; diacylglycerol; Fas; granulosa cells; phospholipase D;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Han, JS Hanyang Univ, Inst Biomed Sci, Coll Med, Seoul 133791, South KoreaHanyang Univ Seoul South Korea 133791 Seoul 133791, South Korea
Citazione:
J.H. Kim et al., "Effects of ceramide, the Fas signal intermediate, on apoptosis and phospholipase D activity in mouse ovarian granulosa cells in vitro", IUBMB LIFE, 48(4), 1999, pp. 445-452

Abstract

Although recent studies have demonstrated that ovarian follicular atresia occurs by apoptosis of granulosa cells, the intracellular signaling pathways involved in apoptotic cell death are still poorly characterized, We examined the role of ceramide as a candidate intracelluar mediator of Fas mediated signaling in cultured granulosa cells. Expression of Fas antigen was demonstrated by Western blot of granulosa cell lysates and immunostaining of cultured granulosa cells, Exposure of granulosa cells to anti-Fas monoclonalantibody (anti-Fas mAb) resulted in significant sphingomyelin hydrolysis, which was accompanied by a progressive increase in endogenous levels of ceramide, The addition of exogenous C-6-ceramide induced drastic morphologicalchange, including nuclear fragmentation and typical apoptotic DNA degradation. Furthermore, both anti-Fas mAb and C-6-ceramide decreased phospholipase D (PLD) activity and diacylglycerol (DAG) concentrations in a time- or a dose-dependent manner, In addition, treatment with phorbol 12-myristate 13-acetate completely attenuated the ceramide-induced inhibition of PLD activity and partially suppressed ceramide-induced apoptosis, These results indicate that the Fas/ceramide signaling pathway might play a role in granulosa cell apoptosis and suggest that the PLD/DAG pathway might be cross-linked to the Fas/ceramide pathway in apoptotic processes of granulosa cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 00:10:51