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Titolo:
Epitopes and functional responses defined by a panel of anti-Fas (CD95) monoclonal antibodies
Autore:
Komada, Y; Inaba, H; Li, QS; Azuma, E; Zhou, YW; Yamamoto, H; Sakurai, M;
Indirizzi:
Mie Univ, Sch Med, Dept Pediat, Mie, Japan Mie Univ Mie JapanMie Univ, Sch Med, Dept Pediat, Mie, Japan Nagoya Univ, Sch Med, Dept Immunol, Aichi, Japan Nagoya Univ Aichi Japan agoya Univ, Sch Med, Dept Immunol, Aichi, Japan Natl Mie Chuo Hosp, Dept Pediat, Mie, Japan Natl Mie Chuo Hosp Mie Japan atl Mie Chuo Hosp, Dept Pediat, Mie, Japan
Titolo Testata:
HYBRIDOMA
fascicolo: 5, volume: 18, anno: 1999,
pagine: 391 - 398
SICI:
0272-457X(199910)18:5<391:EAFRDB>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; CELL-SURFACE ANTIGEN; AUTOIMMUNE LYMPHOPROLIFERATIVE SYNDROME; MEDIATED CYTOTOXICITY; MOLECULAR-CLONING; LIGAND; DEATH; APOPTOSIS; EXPRESSION; MUTATIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Komada, Y Mie Univ, Sch Med, Dept Pediat, Mie, Japan Mie Univ Mie JapanMie Univ, Sch Med, Dept Pediat, Mie, Japan
Citazione:
Y. Komada et al., "Epitopes and functional responses defined by a panel of anti-Fas (CD95) monoclonal antibodies", HYBRIDOMA, 18(5), 1999, pp. 391-398

Abstract

Fas (CD95) is a cell surface glycoprotein that mediates apoptotic cell death when cross-linked with agonistic anti-Fas monoclonal antibodies (MAbs) or the endogenous Fas ligand, In this study, we investigated the in vitro biological properties of a panel of anti-human Fas MAbs, We found that five anti-Fas MAbs of IgG(1) subclass (B.E28, B.G30, B.L25, DX2, and B.G34) induced marked apoptotic cell death in Fas-expressing leukemia cells, although this killing was delayed when compared to the cytolytic effect mediated by the prototypic anti-Fas MAb of IgM subclass (clone CH-11). On the other hand, four clones (ZB4, B.G27, B.D29, and B.K14) efficiently blocked apoptotic cell death induced by the CH-11 MAb or Fas ligand, The ability of these MAbs to inhibit cell death appeared to correlate with their relative affinity for the Fas molecule, Furthermore, different clones recognized the same epitope and elicited different effects (induction or inhibition of cell killing); conversely, different clones elicited the same effect but recognized different epitopes, These results suggest that the different biological effects of anti-Fas MAbs would not be mediated in an epitope-restricted manner. The relative binding affinity might correlate to some extent with the biological properties of the MAb.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:54:45