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Titolo:
Genome stability of Bacillus thuringiensis subsp israelensis isolates
Autore:
Ankarloo, J; Caugant, DA; Ankarloo, J; Caugant, DA; Hansen, BM; Berg, A; Kolsto, AB; Lovgren, A;
Indirizzi:
Univ Stockholm, Dept Microbiol, S-10691 Stockholm, Sweden Univ Stockholm Stockholm Sweden S-10691 obiol, S-10691 Stockholm, Sweden Natl Inst Publ Hlth, Dept Bacteriol, N-0403 Oslo, Norway Natl Inst Publ Hlth Oslo Norway N-0403 pt Bacteriol, N-0403 Oslo, Norway Natl Environm Res Inst, Dept Marine Ecol & Microbiol, DK-4000 Roskilde, Denmark Natl Environm Res Inst Roskilde Denmark DK-4000 K-4000 Roskilde, Denmark Univ Oslo, Ctr Biotechnol, Dept Microbiol, N-0316 Oslo, Norway Univ Oslo Oslo Norway N-0316 echnol, Dept Microbiol, N-0316 Oslo, Norway
Titolo Testata:
CURRENT MICROBIOLOGY
fascicolo: 1, volume: 40, anno: 2000,
pagine: 51 - 56
SICI:
0343-8651(200001)40:1<51:GSOBTS>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHYSICAL MAP; CEREUS CHROMOSOME; CRYSTAL PROTEINS; STRAINS; GENES; ORGANIZATION; ENTEROTOXIN; DIVERSITY; CLONING; SOIL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Lovgren, A BioInvent Therapeut, Solvegatan 41, S-22370 Lund, Sweden BioInvent Therapeut Solvegatan 41 Lund Sweden S-22370 , Sweden
Citazione:
J. Ankarloo et al., "Genome stability of Bacillus thuringiensis subsp israelensis isolates", CURR MICROB, 40(1), 2000, pp. 51-56

Abstract

Swedish soil isolates biochemically classified as Bacillus thuringiensis subsp. israelensis were further examined for genetic diversity by multilocusenzyme electrophoresis (MLEE), random amplified polymorphic DNA analysis (RAPD), pulse field gel electrophoresis (PFGE), and Southern blotting, and were compared with reference strains. All the tested strains belonging to the Br. israelensis serotype H14 were found to be identical, as judged from the RAPD analysis. MLEE analysis gave a similar result; only one H14 strain was found to differ from the remaining H14 strains by one null allele. PFGEanalysis confirmed a very close relationship between the H14 strains but revealed an SfiI restriction fragment of variable size. Southern blot analyses were carried out with probes for the chromosomally encoded flagellin gene(s) and the plasmid-encoded mosquitocidal toxins. All probes gave similar hybridization patterns in the H14 strains. The mosquito toxin probes hybridized only to the H14 strains, except for one probe hybridizing to strain 6:3, which was originally isolated from the same soil sample as strains 6:11 and 6:12. Because the RAPD, MLEE, and PFGE analyses showed that strain 6:3 appears to be unrelated to strains 6:11 and 6.12, the presence of a mosquito toxin sequence in strain 6:3 may suggest that gene transfer has occurred.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 00:43:43