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Titolo:
Cryopreservation of in vitro grown shoot-tips of Olea europaea L-var. Arbequina
Autore:
Martinez, D; Arroyo-Garcia, R; Revilla, MA;
Indirizzi:
Univ Oviedo, Fac Biol, Dept Biol Organismos & Sistemas, E-33071 Oviedo, Spain Univ Oviedo Oviedo Spain E-33071 ismos & Sistemas, E-33071 Oviedo, Spain Univ Autonoma Madrid, Dept Genet Mol Plantas, CSIC, CNB, E-28049 Madrid, Spain Univ Autonoma Madrid Madrid Spain E-28049 IC, CNB, E-28049 Madrid, Spain
Titolo Testata:
CRYO-LETTERS
fascicolo: 1, volume: 20, anno: 1999,
pagine: 29 - 36
SICI:
0143-2044(199901/02)20:1<29:COIVGS>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENCAPSULATION-DEHYDRATION; THERMAL-ANALYSIS; SOMATIC EMBRYOS; LIQUID-NITROGEN; IN-VITRO; DESICCATION; INVITRO; PLANTLETS; MANNITOL; CULTURES;
Keywords:
apices; dehydration; differential scanning calorimetry; liquid nitrogen; olive tree; sucrose preculture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Revilla, MA Univ Oviedo, Fac Biol, Dept Biol Organismos & Sistemas, E-33071 Oviedo, Spain Univ Oviedo Oviedo Spain E-33071 emas, E-33071 Oviedo, Spain
Citazione:
D. Martinez et al., "Cryopreservation of in vitro grown shoot-tips of Olea europaea L-var. Arbequina", CRYO-LETT, 20(1), 1999, pp. 29-36

Abstract

A simple cryopreservation method is described for olive in vitro grown apical shoot-tips. It involves preculturing the meristematic dome with 1 or 2 pairs of leaf primordia for 2 days in culture medium enriched with 0.75 M sucrose, followed by dehydration in the air current of a flow cabinet for 2 h (30% moisture content), transference to cryovials, plunging into liquid nitrogen for storage and rewarming at room temperature. A progressive decrease in survival was achieved both for control and frozen samples as the dehydration period was increased Shoot recovery rates were around 30% for driedand frozen samples which was equivalent to 77% of those surviving dehydration alone. Thermal transitions in samples cryopreserved by this procedure showed absence of enthalpy changes and glass transition phases occurred between -45 and -55 degrees C for cooling and rewarming.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:51:01