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Titolo:
Distribution of lipid and protein in human semen fractions
Autore:
Arienti, G; Saccardi, C; Carlini, E; Verdacchi, R; Palmerini, CA;
Indirizzi:
Ist Biochim & Chim Med, I-06100 Perugia, Italy Ist Biochim & Chim Med Perugia Italy I-06100 Med, I-06100 Perugia, Italy Dipartimento Biol Cellulare & Mol, I-06100 Perugia, Italy Dipartimento Biol Cellulare & Mol Perugia Italy I-06100 0 Perugia, Italy
Titolo Testata:
CLINICA CHIMICA ACTA
fascicolo: 1-2, volume: 289, anno: 1999,
pagine: 111 - 120
SICI:
0009-8981(199911)289:1-2<111:DOLAPI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SEMINAL PLASMA; PREVENTS SPERM; PROSTASOMES; CHOLESTEROL; CAPACITATION; PROGESTERONE; MEMBRANES; PATTERN; AGONIST; FUSION;
Keywords:
semen; prostasomes; lipid; protein; cholesterol; phospholipid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Arienti, G Ist Biochim & Chim Med, Via Giochetto, I-06100 Perugia, Italy Ist Biochim & Chim Med Via Giochetto Perugia Italy I-06100 aly
Citazione:
G. Arienti et al., "Distribution of lipid and protein in human semen fractions", CLIN CHIM A, 289(1-2), 1999, pp. 111-120

Abstract

Human semen is formed by the secretions of different glands. We fractionated semen by centrifugation and obtained four main fractions: (a) spermatozoa, (b) material precipitating at 10 000 X g, (c) prostasomes (precipitate at 105 000 X g), and (d) a soluble fraction. When required, fractions were purified further. We find that most semen protein (about 85%) is in the soluble fraction, 7% in spermatozoa and the remainder is scattered in the otherfractions. We compared the electrophoretic pattern of soluble protein withthe protein of prostasomes and found marked differences. On the other hand, prostasomes, that comprises only about 3% of total semen protein, containabout 45% of cholesterol and almost 15% of Lipid phosphorus with a cholesterol to phospholipid molar ratio greater than 2. On the contrary, phospholipid is largely bound to the fraction containing spermatozoa (about 46% of total lipid phosphorus). This fraction is poor in cholesterol and has a cholesterol to phospholipid molar ratio of about 0.2. The distribution of lipidphosphorus among Lipid classes shows some similarity in the soluble fraction and in prostasomes; in both fractions, sphingomyelin is the most abundant phospholipid (about 50%). On the other hand, phosphatidylcholine is the main phospholipid in spermatozoa-enriched fractions (about 35% of total lipid phosphorus). We conclude that the various fractions of seminal plasma obtained by centrifugation differ markedly from each other as to lipid and protein content. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:26:03