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Titolo:
Localization of 28-kDa peroxiredoxin in rat epithelial tissues and its antioxidant properties
Autore:
Novoselov, SV; Peshenko, IV; Popov, VI; Novoselov, VI; Bystrova, MF; Evdokimov, VJ; Kamzalov, SS; Merkulova, MI; Shuvaeva, TM; Lipkin, VM; Fesenko, EE;
Indirizzi:
Russian Acad Sci, Inst Cell Biophys, Moscow 142292, Russia Russian Acad Sci Moscow Russia 142292 ell Biophys, Moscow 142292, Russia Russian Acad Sci, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow 117871, Russia Russian Acad Sci Moscow Russia 117871 oorgan Chem, Moscow 117871, Russia
Titolo Testata:
CELL AND TISSUE RESEARCH
fascicolo: 3, volume: 298, anno: 1999,
pagine: 471 - 480
SICI:
0302-766X(199912)298:3<471:LO2PIR>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
THIOL-SPECIFIC ANTIOXIDANT; MURINE ERYTHROLEUKEMIA-CELLS; ATP-DEPENDENT PROTEASE; OLFACTORY EPITHELIUM; GLUTATHIONE-PEROXIDASE; THIOREDOXIN PEROXIDASE; SECRETORY PROTEIN; GENE; CLONING; DIFFERENTIATION;
Keywords:
antioxidant; peroxiredoxin; redox; olfactory epithelium; respiratory epithelium; rat (Wistar);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Novoselov, VI Russian Acad Sci, Inst Cell Biophys, Moscow 142292, Russia Russian Acad Sci Moscow Russia 142292 oscow 142292, Russia
Citazione:
S.V. Novoselov et al., "Localization of 28-kDa peroxiredoxin in rat epithelial tissues and its antioxidant properties", CELL TIS RE, 298(3), 1999, pp. 471-480

Abstract

Peroxiredoxins are a novel family of antioxidant proteins that specifically prevent enzymes from metal-catalyzed oxidation. The localization of a member of the mono-cystein subfamily of peroxiredoxins, the 28-kDa protein, indifferent rat tissues and its antioxidant properties were investigated. Byimmunoblotting, the 28-kDa peroxiredoxin was found to be most highly concentrated in olfactory epithelium and present in all tissues tested (skin, lung, trachea, kidney, womb, and brain). Immunostaining with rabbit polyclonal antibody raised against the 28-kDa peroxiredoxin revealed the particularly high level of the 28-kDa peroxiredoxin immunoreactivity in air-contactingareas (apical regions and mucus Of the olfactory and respiratory epithelium and skin epidermis), which are continually exposed to numerous air-borne reactive oxygen species. In the apical regions of the olfactory and respiratory epithelium. the 28kDa-peroxiredoxin immunogold labeling outlined microvilli and cilia and was mainly located in sustentacular cells and in respiratory and goblet cells, as electronmicroscopic analysis revealed. In skin epidermis, the 28-kDa peroxiredoxin immunoreactivity was confined to the granular layer and specifically concentrated in sebaceous glands of hair follicle. In situ hybridization with P-33-labeled antisense RNA probe revealed the expression of the 28-kDa peroxiredoxin mRNA in tissues with a high levelof the 28-kDa peroxiredoxin immunoreactivity. Immunodepletion of the 28-kDa peroxiredoxin profoundry decreased the antioxidant activity of the olfactory tissue extract.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 20:38:49