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Titolo:
Human cultured dendritic cells show differential sensitivity to chemotherapy agents as assessed by the MTS assay
Autore:
Chao, D; Bahl, P; Houlbrook, S; Hoy, L; Harris, AL; Austyn, JM;
Indirizzi:
John Radcliffe Hosp, Nuffield Dept Surg, Oxford OX3 9DU, England John Radcliffe Hosp Oxford England OX3 9DU Surg, Oxford OX3 9DU, England Churchill Hosp, Imperial Canc Res Fund, Med Oncol Unit, Oxford OX3 7LJ, England Churchill Hosp Oxford England OX3 7LJ ncol Unit, Oxford OX3 7LJ, England
Titolo Testata:
BRITISH JOURNAL OF CANCER
fascicolo: 8, volume: 81, anno: 1999,
pagine: 1280 - 1284
SICI:
0007-0920(199912)81:8<1280:HCDCSD>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN BLOOD; PHARMACOKINETICS; CANCER; GENERATION; EXPRESSION;
Keywords:
human; dendritic cell; chemotherapy; MTS; immunotherapy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Chao, D John Radcliffe Hosp, Nuffield Dept Surg, Oxford OX3 9DU, England John Radcliffe Hosp Oxford England OX3 9DU ford OX3 9DU, England
Citazione:
D. Chao et al., "Human cultured dendritic cells show differential sensitivity to chemotherapy agents as assessed by the MTS assay", BR J CANC, 81(8), 1999, pp. 1280-1284

Abstract

Assessment of the chemosensitivity of dendritic cells (DC) may allow more rational development of combined chemotherapy and immunotherapy protocols. Human monocyte-derived DC generated reproducible results in the MTS (Owen'sreagent) assay, which was then used to study DC survival after treatment with four different chemotherapy agents. DC preparations from three different donors were used per drug. DC were sensitive to doxorubicin (concentration range 0.1-50 mu M) with variation in sensitivity between donors (IC50 244-1100 nM). The most extreme variation was seen for vinblastine (concentration range 250-0.025 mu M with IC50 0.15-17.25 mu M). In contrast, there was relative resistance to etoposide (concentration range 0.2-200 mu M) and 5-fluorouracil (concentration range 0.7-7700 mu M) with no toxicity seen until50 mu M and 770 mu M respectively The function of DC in allogeneic mixed leucocyte reactions closely paralleled results from the MTS assays. The differential sensitivity to chemotherapy agents did not appear to be due to expression of P-glycoprotein, These results suggest that etoposide or 5-fluorouracil is less likely to reduce the immunotherapeutic potential of DC and may be valuable in the design of prodrug activation therapy. (C) 1999 CancerResearch Campaign.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 08:56:03