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Titolo:
Precursor and cofactor as a check valve for cephamycin biosynthesis in Streptomyces clavuligerus
Autore:
Khetan, A; Malmberg, LH; Kyung, YS; Sherman, DH; Hu, WS;
Indirizzi:
Univ Minnesota, Dept Chem Engn & Mat Sci, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 at Sci, Minneapolis, MN 55455 USA Univ Minnesota, Dept Microbiol, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 robiol, Minneapolis, MN 55455 USA Univ Minnesota, Biol Proc Technol Inst, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 l Inst, Minneapolis, MN 55455 USA
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 6, volume: 15, anno: 1999,
pagine: 1020 - 1027
SICI:
8756-7938(199911/12)15:6<1020:PACAAC>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYSINE EPSILON-AMINOTRANSFERASE; CEPHALOSPORIN PRODUCTION; C SYNTHESIS; PURIFICATION; SYNTHETASE; ENZYME; DEHYDROGENASE; PENICILLIN; GENES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Hu, WS Univ Minnesota, Dept Chem Engn & Mat Sci, 421 Washington Ave SE, Minneapolis, MN 55455 USA Univ Minnesota 421 Washington Ave SE Minneapolis MNUSA 55455 5 USA
Citazione:
A. Khetan et al., "Precursor and cofactor as a check valve for cephamycin biosynthesis in Streptomyces clavuligerus", BIOTECH PR, 15(6), 1999, pp. 1020-1027

Abstract

The biosynthesis of secondary metabolites is closely linked to primary metabolism via the supply of precursors, cofactors, and cellular energy. The availability of these precursors and cofactors can potentially be rate-limiting for secondary metabolism. A combined experimental and kinetic modeling approach was used to examine the regulation of flux in the cephamycin biosynthetic pathway in Streptomyces clavuligerus. The kinetic parameters of lysine 6-aminotransferase (LAT), the first enzyme leading to cephamycin biosynthesis and one which was previously identified as being a rate-limiting enzyme, were characterized. LAT converts lysine to alpha-aminoadipic acid using alpha-ketoglutarate as a cosubstrate. The K-m values for lysine and alpha-ketoglutarate were substantially higher than those for their intracellularconcentrations, suggesting that lysine and alpha-ketoglutarate may play a key role in regulating the flux of cephamycin biosynthesis. The important role of this precursor/cosubstrate was supported by simulated results using a kinetic model. When the intracellular concentrations and high K-m values were taken into account, the predicted intermediate concentration was similar to the experimental measurements. The results demonstrate the controlling roles that precursors and cofactors may play in the biosynthesis of secondary metabolites.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 02:12:44