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Titolo:
An unstructured kinetic model of macromolecular metabolism in batch and fed-batch cultures of hybridoma cells producing monoclonal antibody
Autore:
Jang, DJ; Barford, JP;
Indirizzi:
Hong Kong Univ Sci & Technol, Dept Chem Engn, Hong Kong, SAR, Peoples R China Hong Kong Univ Sci & Technol Hong Kong SAR Peoples R China oples R China Univ Sydney, Dept Chem Engn, Sydney, NSW 2006, Australia Univ Sydney Sydney NSW Australia 2006 m Engn, Sydney, NSW 2006, Australia
Titolo Testata:
BIOCHEMICAL ENGINEERING JOURNAL
fascicolo: 2, volume: 4, anno: 2000,
pagine: 153 - 168
SICI:
1369-703X(200001)4:2<153:AUKMOM>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONTINUOUS SUSPENSION-CULTURE; SERUM-FREE MEDIUM; STRUCTURED MODEL; CYCLE MODEL; GROWTH-RATE; IMMUNOGLOBULIN; OPTIMIZATION; SIMULATION;
Keywords:
unstructured model; hybridoma; monoclonal antibody; growth phase; macromolecule;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Barford, JP Hong Kong Univ Sci & Technol, Dept Chem Engn, Hong Kong, SAR, Peoples R China Hong Kong Univ Sci & Technol Hong Kong SAR Peoples R China a
Citazione:
D.J. Jang e J.P. Barford, "An unstructured kinetic model of macromolecular metabolism in batch and fed-batch cultures of hybridoma cells producing monoclonal antibody", BIOCH ENG J, 4(2), 2000, pp. 153-168

Abstract

Growth profiles of the batch and fed-batch culture of hybridoma cells producing monoclonal antibody were simulated using an unstructured model. The model describes the production of cellular macromolecules and monoclonal antibody, the metabolism of glucose and glutamine with the production of lactate and ammonia, and the profiles of cell growth in batch and fed-batch culture. Equations describing the cells arrested in G1 phase [T.I. Linardos, N. Kalogerakis, L.A. Behie, Biotechnol. Bioeng. 40 (1992) 359-368; E. Suzuki,D.F Ollis, Biotechnol. Bioeng. 34 (1989) 1398-1402] were included in this model to describe the increase of the specific antibody productivity in thenear-zero specific growth rate, which was observed in the recent experiments in fed-batch cultures of this study and the semi-continuous culture of hybridoma cells [S. Reuveny, D. Velez, L. Miller, J.D. Macmillan, J. Immnol. Methods 86 (1986) 61-69]. This model predicted the increase of specific antibody production rate and the decline of the specific production rate of cellular macromolecules such as DNA, RNA, protein, and polysaccharide in thelate exponential and decline phase of batch culture and at lower specific growth rates in the fed-batch culture. (C) 2000 Elsevier Science S.A. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 08:22:23